WB, IHC-Bond, IHC-P, IF-IC
H
Endogenous
133
Rabbit IgG
#O43490
8842
Product Information
Product Usage Information
This product is the carrier free version of product #64326. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN, or CUT&Tag assays. If you require a carrier-free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the 1st extracellular loop of human CD133 protein. The epitope has been mapped to a region spanning amino acid residues 303-312. This antibody is not sensitive to the glycosylation status of CD133.
Background
CD133, also known as Prominin, was first described as a cell surface marker recognized by monoclonal antibody AC133 on putative hematopoietic stem cells (1). Subsequent cDNA cloning indicated that CD133 is a five-transmembrane protein with a predicted molecular weight of 97 kDa. Due to heavy glycosylation, its apparent molecular weight is 130 kDa as determined by SDS-PAGE analysis (2). Besides blood stem cells, CD133 is expressed on and used to isolate other stem cells, including cancer stem cells (3-7). A deletion mutation in CD133 produces aberrant protein localization and may result in retinal degeneration in humans (8).
- Yin, A.H. et al. (1997) Blood 90, 5002-12.
- Miraglia, S. et al. (1997) Blood 90, 5013-21.
- Handgretinger, R. et al. (2003) Ann N Y Acad Sci 996, 141-51.
- Monzani, E. et al. (2007) Eur J Cancer 43, 935-46.
- O'Brien, C.A. et al. (2007) Nature 445, 106-10.
- Ricci-Vitiani, L. et al. (2007) Nature 445, 111-5.
- Singh, S.K. et al. (2004) Nature 432, 396-401.
- Maw, M.A. et al. (2000) Hum. Mol. Genet. 9, 27-34.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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