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88280
CD16/CD32 (2.4G2) Rat mAb
Primary Antibodies
Monoclonal Antibody

CD16/CD32 (2.4G2) Rat mAb #88280

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CD16/CD32 (2.4G2) Rat mAb: Image 1

To Purchase # 88280S
Product # Size Price
88280S
100 µg $ 79

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Rat IgG2b

Application Key:

  • W-Western
  • IP-Immunoprecipitation
  • IHC-Immunohistochemistry
  • ChIP-Chromatin Immunoprecipitation
  • IF-Immunofluorescence
  • F-Flow Cytometry
  • E-P-ELISA-Peptide

Species Cross-Reactivity Key:

  • H-Human
  • M-Mouse
  • R-Rat
  • Hm-Hamster
  • Mk-Monkey
  • Mi-Mink
  • C-Chicken
  • Dm-D. melanogaster
  • X-Xenopus
  • Z-Zebrafish
  • B-Bovine
  • Dg-Dog
  • Pg-Pig
  • Sc-S. cerevisiae
  • Ce-C. elegans
  • Hr-Horse
  • All-All Species Expected

Product Usage Information

We recommend using 0.25-1.0 μg of antibody per test. The optimal dilution is assay dependent and may require titration.

Application Dilution
Flow Cytometry 1:50 - 1:200

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, pH7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Do not freeze.

Specificity / Sensitivity

CD16/CD32 (2.4G2) Rat mAb recognizes endogenous levels of total CD16/CD32 protein. This antibody detects an epitope within the extracellular domain and is expected to detect all isoforms of CD16 and CD32.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.

Background

CD64 (FcgammaRI), CD32 (FcgammaRII) and CD16 (FcgammaRIII) are three classes of the immunoglobulin superfamily. CD64 has a high affinity for IgG with three Ig-like domains while CD32 and CD16 have low affinities with two Ig-like domains. Two genes encode CD16-A and CD16-B resulting only in a 6 amino acid difference in their ectodomains. However, CD16-A has a transmembrane anchor versus CD16-B, which has a glycosylphosphatidylinositol (1). CD64, CD32 and CD16 are membrane glycoproteins that are expressed by all immunologically active cells and trigger various immune functions (activate B cells, phagocytosis, antibody-dependent cellular cytotoxicity, immune complex clearance and enhancement of antigen presentation) (2). CD16 cross-linking induces tyrosine phosphorylation (Tyr394) of Lck in NK cells (3). CD32 has tyrosine-based activation motifs in the cytoplasmic domain in contrast to CD16, which associates with molecules possessing these motifs (1).

The 2.4G2 clone is widely used as a pre-treatment reagent to reduce non-specific binding of mouse Fc-receptors (4).

  1. Maenaka, K. et al. (2001) J. Biol. Chem. 276, 44898-44904.
  2. Fridman, W. H. et al. (1992) Immunol. Rev. 125, 49-76.
  3. Pignata, C. et al. (1993) J. Immunol. 151, 6794-6800.
  4. Kurlander, R.J. et al. (1984) J Immunol 133, 855-62.

Pathways & Proteins

Explore pathways + proteins related to this product.

Limited Uses

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For Research Use Only. Not For Use In Diagnostic Procedures.
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