WB, IHC-Bond, IHC-P
H Mk
Endogenous
160, 170
Rabbit IgG
#Q86VB7
9332
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:250 - 1:1000 |
Storage
For a carrier free (BSA and azide free) version of this product see product #25121.
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the human CD163 protein.
Background
CD163 is a transmembrane scavenger receptor expressed on the macrophage surface. It has 9 B-type Scavenger Receptor Cysteine-Rich (SRCR) extracellular domains mediating serum haptoglobin clearing/endocytosis, pathogen binding and signal transduction, and calcium binding (1,2). CD163 is used as a surface marker of M2 type macrophages, including M2 type tumor-associated macrophages (TAMs), which facilitate cancer progression by secreting cytokines to promote angiogenesis, immunosuppression, and metastasis (3). Inflammatory stimulation and stress signals can induce extracellular domain shedding of CD163 to generate soluble CD163 (sCD163). The increased sCD163 level in serum is associated with low-grade inflammation in disease conditions (4-7).
- Graversen, J.H. and Moestrup, S.K. (2015) Membranes (Basel) 5, 228-52.
- Etzerodt, A. and Moestrup, S.K. (2013) Antioxid Redox Signal 18, 2352-63.
- Komohara, Y. et al. (2014) Cancer Sci 105, 1-8.
- Zhi, Y. et al. (2017) Mol Med Rep 15, 2931-2939.
- Møller, H.J. et al. (2011) Clin Chem 57, 291-7.
- Grønbaek, H. et al. (2012) Aliment Pharmacol Ther 36, 173-80.
- Aristoteli, L.P. et al. (2006) Atherosclerosis 184, 342-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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