WB
H
Endogenous
48, 65
Rabbit IgG
#Q8N6Q3
57126
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD177 protein.
Background
Cluster of differentiation 177 antigen (CD177, also known as human neutrophil alloantigen 2a and NB1), is a glycosyl-phosphatidylinositol (GPI)-linked N-glycosylated transmembrane protein expressed primarily on the surface of neutrophils (1,2). Expression of CD177 can be induced by granulocyte-colony-stimulating factor and during pregnancy or bacterial infection (3-5). CD177 mediates adhesion and transendothelial migration of neutrophils via interaction with CD31/PECAM-1 and integrin β2 on endothelial cells (6,7). CD177 also promotes the cell surface expression of proteinase 3 (PR3), which may play a role in enhancing endothelial cell junctional integrity and thus vascular integrity during neutrophil diapedesis (8-10). In association with CD11b/ITGAM, integrin β2, and PR3, CD177 promotes activation of TNF-α primed neutrophils including degranulation and superoxide production (11). CD177 is a polymorphic gene that has been linked to several important clinical diseases, including polycythemia vera, Wegener’s granulomatosis, and immune mediated neonatal neutropenia (12-14).
- Matsuo, K. et al. (2000) Transfusion 40, 654-62.
- Xie, Q. et al. (2015) Protein Cell 6, 117-26.
- Stroncek, D.F. et al. (1998) Transfusion 38, 663-8.
- Caruccio, L. et al. (2003) Transfusion 43, 357-63.
- Göhring, K. et al. (2004) Br J Haematol 126, 252-4.
- Sachs, U.J. et al. (2007) J Biol Chem 282, 23603-12.
- Bai, M. et al. (2017) Blood 130, 2092-100.
- von Vietinghoff, S. et al. (2007) Blood 109, 4487-93.
- von Vietinghoff, S. et al. (2008) Clin Exp Immunol 152, 508-16.
- Kuckleburg, C.J. and Newman, P.J. (2013) Arterioscler Thromb Vasc Biol 33, 275-84.
- Jerke, U. et al. (2011) J Biol Chem 286, 7070-81.
- Lalezari, P. et al. (1971) J Clin Invest 50, 1108-15.
- Bettinotti, M.P. et al. (2002) Clin Immunol 102, 138-44.
- Caruccio, L. et al. (2006) Transfusion 46, 441-7.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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