WB, IP, IHC-Bond, IHC-P
H
Endogenous
45-50
Rabbit IgG
#P41217
4345
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
Storage
For a carrier-free (BSA and azide free) version of this product see product #24510.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with human CD200 recombinant protein and reacts with an epitope surrounding Ala164.
Background
CD200 (OX2) and CD200R (OX2R) are membrane glycoprotein members of the Ig superfamily (1-3). CD200 is expressed by a range of cells, including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells, while its receptor, CD200R, is found on myeloid and T cells (1-5). Interaction between CD200 and CD200R downregulates macrophage function and plays a role in immunosuppression and regulation of anti-tumor immune responses (3-7).
- Barclay, A.N. (1981) Immunology 44, 727-36.
- McCaughan, G.W. et al. (1987) Immunogenetics 25, 329-35.
- Wright, G.J. et al. (2000) Immunity 13, 233-42.
- Hoek, R.M. et al. (2000) Science 290, 1768-71.
- Wright, G.J. et al. (2003) J Immunol 171, 3034-46.
- Kretz-Rommel, A. et al. (2007) J Immunol 178, 5595-605.
- Fallarino, F. et al. (2004) J Immunol 173, 3748-54.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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