WB, IP
H M R
Endogenous
55
Rabbit IgG
#P26842
939
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu249 of human CD27 protein.
Background
CD27 (TNFRSF7) is a transmembrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) (1,2). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells (3,4). It is constitutively expressed on naïve CD4 and CD8 T cells and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28). While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation (5,6). Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation, CD27 is shed from the cell surface and soluble CD27 is used as a marker of T cell activation (7,8).
- So, T. et al. (2006) Int J Hematol 83, 1-11.
- Waight, J.D. et al. (2017) Hum Antibodies 25, 87-109.
- Agematsu, K. (2000) Histol Histopathol 15, 573-6.
- Bouaziz, J.D. et al. (2012) Curr Mol Med 12, 519-27.
- Croft, M. Cytokine Growth Factor Rev 14, 265-73.
- Acuto, O. and Michel, F. (2003) Nat Rev Immunol 3, 939-51.
- Lens, S.M. et al. (1998) Semin Immunol 10, 491-9.
- van de Ven, K. and Borst, J. (2015) Immunotherapy 7, 655-67.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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