Revision 1

#15518

Store at -20C

CST Logo
Orders:

877-616-CELL (2355)

[email protected]

Support:

877-678-TECH (8324)

3 Trask Lane | Danvers | Massachusetts | 01923 | USA

For Research Use Only. Not for Use in Diagnostic Procedures.

Applications:
W, IF-F, IF-IC

Reactivity:
M

Sensitivity:
Endogenous

MW (kDa):
80-120

Source/Isotype:
Rabbit IgG

UniProt ID:
#Q64314

Entrez-Gene Id:
12490

Product Usage Information

This product is the carrier free version of product #26233. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

Formulation

Supplied in 1X PBS (10 mM Na2HPO4, 3 mM KCl, 2 mM KH2PO4, and 140 mM NaCl (pH 7.8)). BSA and Azide Free.

For standard formulation of this product see product #26233

Storage

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

Specificity/Sensitivity

CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CD34 protein. Nuclear background may be observed in cultured cell lines by immunofluorescence.

Source / Purification

Monoclonal antibody is produced by immunizing animals with mouse CD34 recombinant protein.

Background

CD34 is a type I transmembrane glycophosphoprotein expressed by hematopoietic stem/progenitor cells, vascular endothelium and some fibroblasts (1). CD34 expression has been the hallmark used to identify hematopoietic stem cells for many years. CD34+ hematopoietic stem cells expand and differentiate into all the lymphohematopoietic lineages upon cytokine or growth factor stimulation and lose CD34 expression upon differentiation. However, recent studies performed in various laboratories conflict with that convention (2). The extracellular domain of CD34 is homologous to CD43, a protein involved in cell-cell adhesion, and CD34 has been shown to function as a negative regulator of cell adhesion (3). CD34 associates with CrkL but not CrkII, is a substrate for PKC, and activation of PKC is coupled with surface expression of CD34 (1,4).

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Applications Key

W: Western Blotting IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)

Cross-Reactivity Key

M: Mouse

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Western blot analysis of extracts from various cell lines using CD34 (E2J1K) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 1: CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD34 protein (mCD34-Myc/DDK; +), using CD34 (E2J1K) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower). Data were generated using the standard formulation of this product.
Western Blotting Image 2: CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse pancreas using CD34 (E2J1K) Rabbit mAb (left, red). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #76113 (right, green pseudocolor) and ProLong® Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.

Revision 1

Cell Signaling Technology Logo
Confocal immunofluorescent analysis of fixed frozen mouse testes using CD34 (E2J1K) Rabbit mAb (left, red). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #76113 (right, green pseudocolor) and ProLong® Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 2: CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)
Confocal immunofluorescent analysis of fixed frozen mouse colon at low magnification (left) and high magnification (right) using CD34 (E2J1K) Rabbit mAb (top, red). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to colabeling with COL1A1 (E8F4L) XP® Rabbit mAb (Alexa Fluor®488 Conjugate) #28368 (bottom, green) and ProLong® Gold Antifade Reagent with DAPI #8961 (bottom, blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 3: CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)
Confocal immunofluorescent analysis of CT26.WT cells (left, positive) and Neuro-2a cells (right, negative) using CD34 (E2J1K) Rabbit mAb (green) and ProLong® Gold Antifade Reagent with DAPI #8961 (blue). Data were generated using the standard formulation of this product.
Immunofluorescence Image 1: CD34 (E2J1K) Rabbit Monoclonal Antibody (BSA and Azide Free)
Orders: 877-616-CELL (2355) [email protected] Support: 877-678-TECH (8324) [email protected] Web: cellsignal.com
For Research Use Only. Not for Use in Diagnostic Procedures.