WB, IP, IHC-P, IF-F, IF-IC
M R
Endogenous
45
Rabbit IgG
#P56528
12494
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Frozen) | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #64666.
Specificity / Sensitivity
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro298 of mouse CD38 protein.
Background
Cyclic ADP-ribose hydrolase 1 (CD38) is a transmembrane protein involved in several important biological processes, including immune response, insulin secretion, and social behavior. Originally described as a glycosylated immune cell surface marker, additional research determined that CD38 is a multifunctional enzyme that catalyzes the synthesis and hydrolysis of cyclic ADP ribose (cADPR) from NAD (1,2). Under acidic conditions, CD38 also catalyzes the synthesis of nicotinic acid adenine dinucleotide phosphate (NAADP) from NADP+. Both cADPR and NAADP act as calcium ion mobilizing messengers that target different intracellular Ca2+ stores (3-6). Since CD38 is the primary mammalian NAD+ glycohydrolase responsible for NAD+ metabolism, CD38 may be a valuable therapeutic target for treatment of metabolic diseases regulated by NAD+-dependent pathways (7,8). CD38 has also been considered a possible therapeutic target for antibody-mediated therapy for myeloma and chronic lymphocytic leukemia (9-11).
- Malavasi, F. et al. (2008) Physiol Rev 88, 841-86.
- Jin, D. et al. (2007) Nature 446, 41-5.
- Lee, H.C. et al. (1999) Mol Cell Biochem 193, 89-98.
- Calcraft, P.J. et al. (2009) Nature 459, 596-600.
- Ogunbayo, O.A. et al. (2011) J Biol Chem 286, 9136-40.
- Lee, H.C. (2012) J Biol Chem 287, 31633-40.
- Cantó, C. et al. (2012) Cell Metab 15, 838-47.
- Escande, C. et al. (2013) Diabetes 62, 1084-93.
- Malavasi, F. et al. (2011) Blood 118, 3470-8.
- Deaglio, S. et al. (2010) Semin Cancer Biol 20, 416-23.
- Chillemi, A. et al. (2013) Mol Med 19, 99-108.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-F: Immunofluorescence (Frozen) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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