WB, IP, IHC-P
H
Endogenous
25-30 (membrane bound); 17 (soluble)
Rabbit IgG
#P29965
959
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
For a carrier free (BSA and azide free) version of this product see product #73222.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CD40 ligand protein.
Background
CD40 ligand (CD40L), also known as CD154, TRAP, and gp39, is the ligand for the TNF receptor family member CD40 (1-6). CD40L is expressed either as a soluble cytokine or as a homotrimeric transmembrane protein. CD40L primarily expressed on the surface of T-cells, but has also been reported in blood platelets, mast cells, basophils, NK cells, and B-cells. It plays an important role in stimulating B-cell cell proliferation and survival and promotes immunoglobulin class switching and secretion of IgE (7). Signals generated by CD40 vary depending on cell type and include activation of MAPK pathways as well as NF-κB (8-11). Mutations within the CD40L gene are associated with X-linked hyper-IgM syndrome characterized by high serum levels of IgM and decreased levels of other isotypes (12). The CD40L/CD40 pathway is an important area of interest in the study of cancer, vascular diseases, and inflammatory disorders (13-15).
- Noelle, R.J. et al. (1992) Proc Natl Acad Sci USA 89, 6550-4.
- Graf, D. et al. (1992) Eur J Immunol 22, 3191-4.
- Armitage, R.J. et al. (1992) Nature 357, 80-2.
- Hollenbaugh, D. et al. (1992) EMBO J 11, 4313-21.
- Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66.
- Castle, B.E. et al. (1993) J Immunol 151, 1777-88.
- Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50.
- Li, Y.Y. et al. (1996) J Immunol 157, 1440-7.
- Pearson, L.L. et al. (2001) Int Immunol 13, 273-83.
- Berberich, I. et al. (1996) EMBO J 15, 92-101.
- Aruffo, A. et al. (1993) Cell 72, 291-300.
- Berberich, I. et al. (1994) J Immunol 153, 4357-66.
- Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57.
- Hassan, G.S. et al. (2012) Immunobiology 217, 521-32.
- Zhang, B. et al. (2013) Immunol Lett 153, 58-61.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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