WB, IHC-P
H Mk
Endogenous
50-70
Rabbit IgG
#P15529
4179
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:800 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro199 of human CD46 protein.
Background
Complement Regulatory Protein; Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system (1). CD46 exhibits a cofactor activity that promotes inactivation of C3b and C4b by serum factor 1, thereby protecting host (self) cells from complement-dependent cytotoxicity (1,2). The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation (2,3). In addition to its role in complement inactivation, CD46 can function as a receptor for selected bacteria and viruses (4), and is reportedly required for proper fusion of spermatozoa to the oocyte membrane during fertilization (5). CD46 is implicated in the development and/or progression of selected cancer types. For example, research studies show elevated CD46 expression in medulloblastoma tumor samples (6), while CD46 expression has been linked with poor prognosis in breast cancer (7). It has been suggested that upregulation of CD46 may serve to protect cancer cells from complement-dependent cytotoxicity, thereby evading destruction by the immune system (8,9).
- Liszewski, M.K. et al. (1991) Annu Rev Immunol 9, 431-55.
- Riley-Vargas, R.C. et al. (2004) Trends Immunol 25, 496-503.
- Noris, M. and Remuzzi, G. (2009) N Engl J Med 361, 1676-87.
- Cattaneo, R. (2004) J Virol 78, 4385-8.
- Taylor, C.T. et al. (1994) Hum Reprod 9, 907-11.
- Studebaker, A.W. et al. (2010) Neuro Oncol 12, 1034-42.
- Maciejczyk, A. et al. (2011) Appl Immunohistochem Mol Morphol 19, 540-6.
- Zhang, S. et al. (2013) FEBS Lett 587, 645-51.
- Cui, W. et al. (2012) FEBS Lett 586, 766-71.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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