WB, IHC-Bond, IHC-P
H
Endogenous
45-50
Rabbit IgG
#P09326
962
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:200 |
| Immunohistochemistry (Paraffin) | 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #90615.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CD48 protein.
Background
CD48 is a glycosylphosphatidylinositol (GPI) -anchored membrane protein of the signaling lymphocyte activation molecule (SLAM) family, also known as SLAMF2 and BLAST-1. It is constitutively expressed on most hematopoietic cells (not on neutrophils and a subset of long-term hematopoietic stem cells in mice) and can be upregulated under certain conditions like infection (1). Interaction with its low affinity ligand CD2 promotes adhesion and TCR signaling (2,3). Interaction with the high affinity ligand CD244 (2B4) regulates natural killer (NK) and CD8 T cell activation and cytolytic function (4-6).
- McArdel, S.L. et al. (2016) Clin Immunol 164, 10-20.
- Moran, M. and Miceli, M.C. (1998) Immunity 9, 787-96.
- Muhammad, A. et al. (2009) J Immunol 182, 7672-80.
- Assarsson, E. et al. (2005) Mol Immunol 42, 419-23.
- Assarsson, E. et al. (2005) J Immunol 175, 2045-9.
- Elishmereni, M. and Levi-Schaffer, F. (2011) Int J Biochem Cell Biol 43, 25-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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