WB, IHC-Bond, IHC-P
H
Endogenous
140
Rabbit IgG
#P16109
6403
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:400 - 1:1600 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #31214.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu433 of human CD62P/P-Selectin protein.
Background
CD62P/P-Selectin is a type-I transmembrane glycoprotein that is largely expressed and stored in intracellular granules by resting platelets, megakaryocytes, and vascular endothelial cells (1,2). The restricted expression pattern of CD62P/P-Selectin positions this molecule as a key regulator of hemostasis, thrombosis, and inflammation. Platelets and endothelial cells that become activated in response to proinflammatory cytokines express CD62P/P-Selectin on the cell surface, which serves as an adhesion receptor to facilitate recruitment of leukocytes to sites of inflammation (3-5). CD62P/P-Selectin has also been implicated in cancer cell metastasis by promoting the adhesion of tumor cells in circulation to activated platelets and endothelial cells (6,7).
- Stenberg, P.E. et al. (1985) J Cell Biol 101, 880-6.
- Johnston, G.I. et al. (1989) J Biol Chem 264, 1816-23.
- Pouyani, T. and Seed, B. (1995) Cell 83, 333-43.
- Somers, W.S. et al. (2000) Cell 103, 467-79.
- Hattori, R. et al. (1989) J Biol Chem 264, 7768-71.
- Erpenbeck, L. and Schön, M.P. (2010) Blood 115, 3427-36.
- Köhler, S. et al. (2010) Br J Cancer 102, 602-9.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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