WB
H
Endogenous
40-45
Rabbit IgG
#P21854
971
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn215 of human CD72 protein.
Background
CD72 is an inhibitory co-receptor of the B cell antigen receptor (BCR) and is expressed primarily on B cells (1,2). The cytoplasmic domain of CD72 contains immunoreceptor tyrosine-based inhibition motifs (ITIMs) that are phosphorylated by Lyn when Lyn associates with BCR (3-5). This phosphorylation leads to recruitment of SHP-1 to CD72 and negative regulation of BCR signaling (4,5). The extracellular portion of CD72 contains a C-type lectin domain, and binding its ligand, CD100/Semaphorin-4D, leads to dissociation of CD72 and SHP-1 and thus relief of BCR signaling suppression (6,7). Recently, Semaphorin-3A was also reported to act as a ligand for CD72; however, unlike CD100/Semaphorin-4D, association with Semaphorin-3A induces CD72 ITAM phosphorylation and association of CD72 with SHP-1 (8). CD72 also plays a role in systemic lupus erythematosus (SLE); through its recognition of the self-antigen Sm/RNP, CD72 suppresses the TLR7-dependent B cell response to nucleic acid self-antigens, inhibiting SLE development (9).
- Wu, Y. et al. (1998) Curr Biol 8, 1009-17.
- Tsubata, T. (2018) Front Immunol 9, 2276.
- Thomas, M.L. (1995) J Exp Med 181, 1953-6.
- Adachi, T. et al. (1998) J Immunol 160, 4662-5.
- Tsubata, T. (2012) Infect Disord Drug Targets 12, 181-90.
- Kumanogoh, A. et al. (2000) Immunity 13, 621-31.
- Ishida, I. et al. (2003) Int Immunol 15, 1027-34.
- Eiza, N. et al. (2023) J Autoimmun 134, 102960.
- Tsubata, T. (2019) Immune Netw 19, e1.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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