WB, IP
H
Endogenous
23-65
Rabbit IgG
#Q01151
9308
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro63 of human CD83 protein.
Background
CD83 is a single-transmembrane protein with a calculated molecular weight (MW) of 23 kDa, but due to heavy and differential glycosylation, its apparent MW ranges from 23 to 70 kDa. CD83 is predominantly expressed on mature dendritic cells (DCs) and has been used as a DC activation/maturation marker as its increased expression is correlated with upregulation of HLA class II antigen expression on DCs (1-3). CD83 is also expressed at a low level on lymphocytes and is upregulated upon lymphocyte activation (4, 5). Thymic epithelial cells also express CD83, which is required for normal CD4+ T cell development (6, 7). CD83 is also expressed as a soluble form (sCD83) that can be found in serum of healthy adults (8). sCD83 has been shown to negatively regulate immune response by lymphocytes (9, 10).
- Zhou, L.J. et al. (1992) J Immunol 149, 735-42.
- Prechtel, A.T. and Steinkasserer, A. (2007) Arch Dermatol Res 299, 59-69.
- Prazma, C.M. and Tedder, T.F. (2008) Immunol Lett 115, 1-8.
- Prazma, C.M. et al. (2007) J Immunol 179, 4550-62.
- Lüthje, K. et al. (2008) Int Immunol 20, 949-60.
- Fujimoto, Y. et al. (2002) Cell 108, 755-67.
- Breloer, M. and Fleischer, B. (2008) Trends Immunol 29, 186-94.
- Hock, B.D. et al. (2001) Int Immunol 13, 959-67.
- Lin, W. et al. (2017) Sci Rep 7, 12895.
- Horvatinovich, J.M. et al. (2017) J Immunol 198, 2286-2301.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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