WB, IP
M
Endogenous
100-200
Rabbit IgG
#Q3U0X8
84544
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu368 of mouse CD96 protein.
Background
Cluster of Differentiation 96 (CD96), also known as T cell-activated increased late expression protein (TACTILE), is a heavily glycosylated type I transmembrane protein belonging to the immunoglobulin superfamily (1-3). CD96 is expressed on peripheral T cells and natural killer (NK) cells, and is strongly upregulated on these cell types after activation (1,4). It is also expressed on transformed T cells, and at very low levels on activated B cells (1,5,6). CD96 interacts with the receptor ligand PVR (CD155), and is involved in adhesion of activated T and NK cells to target cells during the late phase of the immune response (2,4). CD96 competes with DNAM-1 (CD226) for binding to PVR (CD155) in order to negatively regulate T and NK cell activity, and thus blockade of CD96 in order to induce anti-tumor immune responses is investigated for potential immunotherapeutic intervention in cancer (7-9). Alternative splicing occurs at this locus, and two transcript variants encoding distinct isoforms with variable functions have been identified (1,10).
- Wang, P.L. et al. (1992) J Immunol 148, 2600-8.
- Seth, S. et al. (2007) Biochem Biophys Res Commun 364, 959-65.
- Georgiev, H. et al. (2018) Front Immunol 9, 1072.
- Fuchs, A. et al. (2004) J Immunol 172, 3994-8.
- Burger, R. et al. (1999) Leuk Res 23, 19-27.
- Gramatzki, M. et al. (1998) Exp Hematol 26, 1209-14.
- Chan, C.J. et al. (2014) Nat Immunol 15, 431-8.
- Blake, S.J. et al. (2016) Cancer Discov 6, 446-59.
- Brooks, J. et al. (2018) Cancer Res 78, 475-88.
- Meyer, D. et al. (2009) J Biol Chem 284, 2235-44.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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