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9868
CDK Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

CDK Antibody Sampler Kit #9868

Citations (12)
Simple Western™ analysis of lysates (0.1 mg/mL) from COS-7 cells using CDK6 (D4S8S) Rabbit mAb #13331. The virtual lane view (left) shows the target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Confocal immunofluorescent analysis of T98G cells (left, positive) or VCaP cells (right, negative) using CDK6 (D4S8S) Rabbit mAb (green), DyLight 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from Hep G2 cells and CDK9 (C12F7) Rabbit mAb using a protocol based on the RBP-eCLIP Kit from EclipseBio. The figure shows binding across the PUM2 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.
Simple Western™ analysis of lysates (1.0 mg/mL) from HeLa cells using CDK2 (78B2) Rabbit mAb #2546. The virtual lane view (left) shows a single target band (as indicated) at 1:10 and 1:50 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:10 (blue line) and 1:50 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.
Immunoprecipitation of CDK7 protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (E7Q5L) mAb IgG2b Isotype Control #53484, and lane 3 is CDK7 (MO1) Mouse mAb. Western blot analysis was performed using CDK7 (E4K4W) Rabbit mAb #42863.
Western blot analysis of extracts from various cell lines using CDK4 (D9G3E) Rabbit mAb.
Western blot analysis of extracts from HeLa, Jurkat, and COS-7 cells using CDK6 (D4S8S) Rabbit mAb.
Western blot analysis of extracts from various cell types using CDK9 (C12F7) Rabbit mAb.
Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells, using CDK2 (78B2) Rabbit mAb.
Western blot analysis of extracts from various cell lines using CDK7 (MO1) Mouse mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from HeLa cells synchronized at various stages of the cell cycle, using cdc2 (POH1) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK4 (D9G3E) Rabbit mAb.
Immunoprecipitation of CDK6 from HeLa cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or CDK6 (D4S8S) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using CDK6 (DCS83) Mouse mAb #3136.
Immunoprecipitation of CDK9 from HeLa cells using CDK9 (C12F7) Rabbit mAb. Western blot detection was performed using the same antibody. Lane 1 is 5% input.
Flow cytometric analysis of Jurkat cells using CDK2 (78B2) Rabbit mAb (right) and Propidium Iodide (PI)/RNase Staining Solution #4087, compared to a secondary only sample (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing nuclear localization, using CDK7 (MO1) Mouse mAb.
Confocal immunofluorescent analysis of HT-29 cells, transfected with SignalSilence® Control siRNA (Unconjugated) #6568 (left), SignalSilence® cdc2 siRNA I #3500 (center) or SignalSilence® cdc2 siRNA II #3600 (right), using cdc2 (POH1) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CDK4 (D9G3E) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using CDK9 (C12F7) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right).
Confocal immunofluorescent analysis of MCF7 cells using CDK4 (D9G3E) Rabbit mAb (green), p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8493 (red), and Phospho-Histone H3 (Ser10) (D2C8) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #3458 (blue pseudocolor).
Immunohistochemical analysis of paraffin-embedded K7M2 mouse syngeneic tumor using CDK9 (C12F7) Rabbit mAb.
Flow cytometric analysis of Jurkat cells using CDK4 (D9G3E) Rabbit mAb (right) and Propidium Iodide (PI)/RNase Staining Solution #4087, compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (left). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Confocal immunofluorescent analysis of HeLa cells using CDK9 (C12F7) Rabbit mAb (green). Actin filaments have been labeled with DY-555 phalloidin (red).
Flow cytometric analysis of HeLa cells using CDK9 (C12F7) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
To Purchase # 9868
Cat. # Size Qty. Price
9868T
1 Kit  (6 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
cdc2 (POH1) Mouse mAb 9116 20 µl
  • WB
  • IP
  • IF
H Mk 34 Mouse IgG2a
CDK2 (78B2) Rabbit mAb 2546 20 µl
  • WB
  • IP
  • F
H M R Mk 33 Rabbit 
CDK4 (D9G3E) Rabbit mAb 12790 20 µl
  • WB
  • IHC
  • IF
  • F
H Mk 30 Rabbit IgG
CDK6 (D4S8S) Rabbit mAb 13331 20 µl
  • WB
  • IP
  • IF
H Mk 36 Rabbit IgG
CDK7 (MO1) Mouse mAb 2916 20 µl
  • WB
  • IP
  • IHC
H R 40 Mouse IgG2b
CDK9 (C12F7) Rabbit mAb 2316 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
  • eCLIP
H M R Hm Mk B Dg 42, 55 Rabbit 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Rab Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
M Horse 

Product Description

The CDK Antbody Sampler Kit provides and economical means of evaluating Cdk proteins. The kit contains enough primary and secondary antibodies to perform two western blot experiments.

Specificity / Sensitivity

Each antibody in the CDK Antibody Sampler Kit detects endogenous levels of its respective target protein and does not cross-react with other family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human cdc2 fusion protein, synthetic peptides corresponding to residues of human CDK2 and recombinant human CDK7, and residues near the carboxy terminus of human CDK4, human CDK6, and human CDK9 proteins.

Background

Cyclin-dependent kinases (CDKs) are the core effectors of cell cycle progression. CDK activity is regulated through association with their cyclin partners and cyclin-dependent kinase inhibitors (CKIs) as well as by activating and inhibitory phosphorylation events. Inhibition is mediated by Wee1 and Myt1 kinases that target residues at the amino terminus of CDK1 (1,2). Dephosphorylation of these residues by cdc25 phosphatase leads to activation of CDK kinase activity (3). The CDK7/cyclinH complex is the ubiquitous mammalian CDK-activating kinase (CAK) that phosphorylates a conserved threonine residue in the T-loop domain of CDKs. The carboxy-terminal domain of RNA polymerase II is also a target of CAK as well as CDK9/cyclinT (4,5). CDK4/6 associate with cyclinD and phosphorylate retinoblastoma protein and initiate progression through the restriction point in G1 (6). CDK2 associates with cyclinE in early S phase and cyclinA later in G2. CDK1/cyclinB regulates the initiation of mitotic events (7).

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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