WB
H M R
Endogenous
40
Rabbit IgG
#Q15131
8558
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val24 of human CDK10 protein.
Background
Cyclin Dependent Kinase 10 (CDK10) is a Cdc2-related protein kinase that binds to and inhibits the transactivation activity of the transcription factor Ets2 (1).
CDK10 is activated by cyclin M, which is mutated and unable to activate CDK10 in the human developmental disorder, STAR syndrome. Phosphorylation of Ets2 by CDK10/Cyclin M leads to degradation of Ets2 by the proteasome (2). CDK10 also plays a role in the development of the zebrafish nervous system (3). Studies have shown that expression of CDK10, which is modulated by promoter hypermethylation, is decreased in human cancer (4-6). Further, studies show that CDK10 expression in breast cancer affects response to tamoxifen (7), and is correlated with disease progression (8). CDK10 regulates the expression of c-RAF, and signaling through the MAPK pathway (2-3, 6-7).
- Kasten, M. and Giordano, A. (2001) Oncogene 20, 1832-8.
- Guen, V.J. et al. (2013) Proc Natl Acad Sci U S A 110, 19525-30.
- Yeh, C.W. et al. (2013) J Biol Chem 288, 27927-39.
- You, Y. et al. (2013) Cell Oncol (Dordr) 36, 323-31.
- Zhong, X.Y. et al. (2012) Gene 498, 68-74.
- Yu, J.H. et al. (2012) Oncol Rep 27, 1266-76.
- Iorns, E. et al. (2008) Cancer Cell 13, 91-104.
- You, Y. et al. (2015) Cell Oncol (Dordr) 38, 485-91.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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