WB, IP, ChIP
H Mk
Endogenous
12
Rabbit IgG
#O14519
8099
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Chromatin IP | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro12 of human CDK2AP1 protein.
Background
Cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) was first identified as a tumor suppressor in oral cancer and is a member of the nucleosome remodeling and histone deacetylase (NuRD) complex (1-4). Also known as p12 or DOC-1, CDK2AP1 binds to unphosphorylated CDK2 and DNA polymerase alpha/primase to negatively regulate their activities (5,6). CDK2AP1 also plays a role in the silencing of Oct-4 during stem cell differentiation (7). Low CDK2AP1 expression in oral cancers is associated with poor prognosis (8). Lower expression has also shown to be resistant to cisplatin treatment (9). In addition to oral cancers, CDK2AP1 plays a role in other tumor types, including colorectal, testicular, and ovarian cancers (10,11).
- Todd, R. et al. (1995) FASEB J 9, 1362-70.
- Daigo, Y. et al. (1997) Genes Chromosomes Cancer 20, 204-7.
- Tsuji, T. et al. (1998) J Biol Chem 273, 6704-9.
- Spruijt, C.G. et al. (2010) Mol Biosyst 6, 1700-6.
- Shintani, S. et al. (2000) Mol Cell Biol 20, 6300-7.
- Matsuo, K. et al. (2000) FASEB J 14, 1318-24.
- Deshpande, A.M. et al. (2009) J Biol Chem 284, 6043-7.
- Shintani, S. et al. (2001) Clin Cancer Res 7, 2776-82.
- Kim, Y. et al. (2005) Oncogene 24, 407-18.
- Yuan, Z. et al. (2003) Oncogene 22, 6304-10.
- Figueiredo, M.L. et al. (2006) Mol Reprod Dev 73, 987-97.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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