Revision 1
#17395
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W, IP
Reactivity:
H M Mk
Sensitivity:
Endogenous
MW (kDa):
53
Source/Isotype:
Rabbit IgG
UniProt ID:
#P49336
Entrez-Gene Id:
1024
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity/Sensitivity
Source / Purification
Background
Mediator complex interactions control various biological processes, including insulin signaling (2), NF-κB-dependent signaling (3), stem cell pluripotency and self renewal (4,5), and proliferation of colon cancer cells (6,7).
CDK8/Cyclin C, along with Med12 and Med13, constitute a subcomplex within the Mediator Complex thought to act as a molecular switch, inhibiting Pol II recruitment and transcription initiation (8,9). Expression of CDK8 abrogates E2F-1-dependent inhibition of β-catenin activity in colon cancer cells (9). High levels of CDK8 coincide with high β-catenin-dependent transcription in colon cancer cells, and their proliferation can be inhibited by suppressing CDK8 expression (8).
CDK8 can phosphorylate Ser727 on STAT1, which reduces natural killer (NK) cell toxicity (10,11). As such, inhibitors are being pursued as potential therapeutics to enhance NK cell activity and combat a variety of cancer types (12,13).
Background References
- Malik, S. and Roeder, R.G. (2005) Trends Biochem Sci 30, 256-63.
- Wang, W. et al. (2009) Dev Cell 16, 764-71.
- van Essen, D. et al. (2009) PLoS Biol 7, e73.
- Tutter, A.V. et al. (2009) J Biol Chem 284, 3709-18.
- Varelas, X. et al. (2008) Nat Cell Biol 10, 837-48.
- Firestein, R. et al. (2008) Nature 455, 547-51.
- Morris, E.J. et al. (2008) Nature 455, 552-6.
- Knuesel, M.T. et al. (2009) Mol Cell Biol 29, 650-61.
- Knuesel, M.T. et al. (2009) Genes Dev 23, 439-51.
- Bancerek, J. et al. (2013) Immunity 38, 250-62.
- Putz, E.M. et al. (2013) Cell Rep 4, 437-44.
- Dale, T. et al. (2015) Nat Chem Biol 11, 973-80.
- Rzymski, T. et al. (2017) Oncotarget , .
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: Human M: Mouse Mk: Monkey
Trademarks and Patents
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