WB, IP
H M R
Endogenous
23
Rabbit IgG
#Q16667
1033
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro107 of human CDKN3 protein.
Background
CDKN3 (Cyclin-Dependent Kinase Inhibitor 3)/KAP (Kinase-Associated Phosphatase) is a dual specificity phosphatase that regulates cell cycle progression through dephosphorylation and inhibition of cyclin-dependent kinases (1). CDKN3 promotes cancer cell proliferation and invasion (2-4), as well as resistance to cisplatin (5,6). Overexpression of CDKN3 is associated with tumor progression and poor prognosis in several human cancers (5-9).
- Hannon, G.J. et al. (1994) Proc Natl Acad Sci U S A 91, 1731-5.
- Deng, M. et al. (2016) Mol Med Rep 14, 1523-30.
- Li, Y. et al. (2017) Oncol Res 25, 721-731.
- Yu, H. et al. (2020) Oncol Lett 19, 542-548.
- Wang, J. et al. (2019) Cancer Manag Res 11, 3253-3264.
- Li, W.H. et al. (2020) Eur Rev Med Pharmacol Sci 24, 3614-3623.
- Dai, W. et al. (2020) J Int Med Res 48, 300060520936879.
- Fan, C. et al. (2015) Br J Cancer 113, 1735-43.
- Barrón, E.V. et al. (2015) PLoS One 10, e0137397.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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