WB
H
Endogenous
200
Rabbit IgG
#P31997
1088
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CEACAM8 protein.
Background
Carcinoembryonic antigen-related cell adhesion molecule 8 (CEACAM8), also known as CD66b, is a member of the CEA-related cell-adhesion molecule (CEACAM) family (1). CEACAMs bind to themselves and other family members to carry out numerous cellular functions, including proliferation, signaling, differentiation, tumor suppression, and survival (2). CEACAM8 is a single-chain, glycosylphosphatidylinositol (GPI)-anchored, highly glycosylated protein that under normal conditions is expressed almost exclusively on granulocytes (3). Heterophilic interactions of CEACAM8 with other surface molecules, such as CEACAM6, have been shown to be involved in regulating cell adhesion in a calcium-independent manner (4). As such, CEACAM8 has demonstrated utility as a marker of neutrophil and eosinophil activation (5,6) and in pathological conditions is shown to be highly expressed in primary myelofibrosis and acute lymphoblastic leukemias (7,8). Assessment of CEACAM8 positive tumor-infiltrating neutrophils has demonstrated value as a prognostic factor in multiple cancer types (9,10).
- Kuespert, K. et al. (2006) Curr Opin Cell Biol 18, 565-71.
- Bonsor, D.A. et al. (2015) Proc Natl Acad Sci U S A 112, 13561-6.
- Zhao, L. et al. (2004) J Immunol Methods 293, 207-14.
- Kuroki, M. et al. (2001) J Leukoc Biol 70, 543-50.
- Yamanaka, T. et al. (1996) Protein Expr Purif 7, 438-46.
- Yoon, J. et al. (2007) J Immunol 179, 8454-62.
- Hasselbalch, H.C. et al. (2011) Leuk Res 35, 1330-4.
- Lasa, A. et al. (2008) Ann Hematol 87, 205-11.
- Waldmann, T.A. (1987) Adv Exp Med Biol 213, 129-37.
- Zhang, H. et al. (2018) Ann Surg 267, 311-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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