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4753
Cellular Localization IF Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cellular Localization IF Antibody Sampler Kit #4753

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Cellular Localization IF Antibody Sampler Kit: Image 1

Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Cellular Localization IF Antibody Sampler Kit: Image 2

Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Cellular Localization IF Antibody Sampler Kit: Image 3

Confocal immunofluorescent analysis of Caki-1 cells using NUP98 (C39A3) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Cellular Localization IF Antibody Sampler Kit: Image 4

Confocal immunofluorescent analysis of HeLa cells using Fibrillarin (C13C3) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Cellular Localization IF Antibody Sampler Kit: Image 5

Flow cytometric analysis of HeLa cells using LC3B (D11) XP® Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Cellular Localization IF Antibody Sampler Kit: Image 6

Confocal immunofluorescent analysis of HeLa cells using Rab5A (E6N8S) Mouse mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Cellular Localization IF Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of HeLa cells using Calnexin (C5C9) Rabbit mAb (green). Actin filaments have been labeled using DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cellular Localization IF Antibody Sampler Kit: Image 8

Flow cytometric analysis of human peripheral blood lymphocytes using Histone H3 (D1H2) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Cellular Localization IF Antibody Sampler Kit: Image 9

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H2A (D6O3A) Rabbit mAb or Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MYT-1 Exon 1 Primers #4493, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Cellular Localization IF Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cellular Localization IF Antibody Sampler Kit: Image 11

Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green) and β-Actin (8H10D10) Mouse mAb #3700 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Cellular Localization IF Antibody Sampler Kit: Image 12

Western blot analysis of cell extracts from various cell types using NUP98 (C39A3) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 13

Western blot analysis of extracts from various cell types using Fibrillarin (C13C3) Antibody.

Cellular Localization IF Antibody Sampler Kit: Image 14

Confocal immunofluorescent analysis of HCT-116 cells, untreated (left) or choroquine-treated (50 uM, overnight; right) using LC3B (D11) XP® Rabbit mAb (green) and β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 555 Conjugate) #5612 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cellular Localization IF Antibody Sampler Kit: Image 15

Immunohistochemical analysis of paraffin-embedded human ductal carcinoma of the breast using Rab5A (E6N8S) Mouse mAb.

Cellular Localization IF Antibody Sampler Kit: Image 16

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Calnexin (C5C9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Cellular Localization IF Antibody Sampler Kit: Image 17

Confocal immunofluorescent analysis of HeLa cells using Histone H3 (D1H2) XP® Rabbit mAb (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

Cellular Localization IF Antibody Sampler Kit: Image 18

Confocal immunofluorescent analysis of HeLa cells using Histone H2A (D6O3A) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Cellular Localization IF Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded human glioblastoma using β-Tubulin (9F3) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 20

Western blot analysis of extracts from various cell lines, untreated (-) or treated overnight with chloroquine (50 μM) (+), using LC3B (D11) XP® Rabbit mAb (upper) or LC3B Antibody #2775 (lower).

Cellular Localization IF Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded human serous papillary carcinoma of the ovary using Rab5A (E6N8S) Mouse mAb.

Cellular Localization IF Antibody Sampler Kit: Image 22

Western blot analysis of extracts from PANC1, HepG2 and A204 cells using Calnexin (C5C9) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Histone H3 (D1H2) XP® Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 24

Western blot analysis of extracts from various cell lines using Histone H2A (D6O3A) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Tubulin (9F3) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 27

Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® LC3B siRNA I #6212 (+) or SignalSilence® LC3B siRNA II #6213 (+), using LC3B (D11) XP® Rabbit mAb #3868 and α-Tubulin (11H10) Rabbit mAb #2125. The LC3B (D11) XP® Rabbit mAb confirms silencing of LC3B expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of LC3B siRNA.

Cellular Localization IF Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using Rab5A (E6N8S) Mouse mAb (left) compared to concentration matched Mouse (G3A1) IgG1 Isotype Control #5415 (right).

Cellular Localization IF Antibody Sampler Kit: Image 29

Western blot analysis of extracts from various cell lines using Histone H3 (D1H2) XP® Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded human melanoma using β-Tubulin (9F3) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 31

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).

Cellular Localization IF Antibody Sampler Kit: Image 32

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Rab5A (E6N8S) Mouse mAb.

Cellular Localization IF Antibody Sampler Kit: Image 33

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).

Cellular Localization IF Antibody Sampler Kit: Image 34

Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.

Cellular Localization IF Antibody Sampler Kit: Image 35

Immunoprecipitation of Rab5A protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, lane 3 is Rab5A (E6N8S) Mouse mAb without HeLa cell extracts, and lane 4 is Rab5A (E6N8S) Mouse mAb. Western blot analysis was performed using Rab5A (E6N8S) Mouse mAb.

Cellular Localization IF Antibody Sampler Kit: Image 36

Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 37

Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.

Cellular Localization IF Antibody Sampler Kit: Image 38

Immunoprecipitation of Rab5A protein from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is Rab5A (E6N8S) Mouse mAb. Western blot analysis was performed using Rab5 (C8B1) Rabbit mAb #3547.

Cellular Localization IF Antibody Sampler Kit: Image 39

Western blot analysis of extracts from various cell lines using Rab5A (E6N8S) Mouse mAb.

To Purchase # 4753T
Product # Size Price
4753T
1 Kit  (9 x 20 µl) $ 609

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
β-Tubulin (9F3) Rabbit mAb 2128 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk Z B 55 Rabbit IgG
COX IV (3E11) Rabbit mAb 4850 20 µl
  • WB
  • IP
  • IHC
  • IF
  • F
H R Mk Z B Pg 17 Rabbit IgG
NUP98 (C39A3) Rabbit mAb 2598 20 µl
  • WB
  • IP
  • IF
H M R Mk 98 Rabbit IgG
Fibrillarin (C13C3) Rabbit mAb 2639 20 µl
  • WB
  • IF
H M R Mk 37 Rabbit IgG
LC3B (D11) XP® Rabbit mAb 3868 20 µl
  • WB
  • IF
  • F
H M R 14, 16 Rabbit IgG
Rab5A (E6N8S) Mouse mAb 46449 20 µl
  • WB
  • IP
  • IHC
  • IF
H M R Mk 25 Mouse IgG1
Calnexin (C5C9) Rabbit mAb 2679 20 µl
  • WB
  • IHC
  • IF
H Mk 90 Rabbit IgG
Histone H3 (D1H2) XP® Rabbit mAb 4499 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 17 Rabbit IgG
Histone H2A (D6O3A) Rabbit mAb 12349 20 µl
  • WB
  • IF
  • ChIP
H M R Mk Z GP 14 Rabbit IgG

Product Description

The Cellular Localization IF Antibody Sampler Kit provides an economical means for identification of cellular organelles by fluorescence immnuocytochemistry (IF-IC). This kit includes enough primary antibody to perform at least twenty IF-IC tests or two Western blots with each antibody.

Specificity / Sensitivity

Each antibody in the Cellular Localization IF Antibody Sampler Kit recognizes only it specific target and does not cross-react with other family members. Each antibody has been validated for IF-IC and stains the organelles indicated above. Expression of these proteins may vary in different cells and tissues. Please see www.cellsignal.com for additional specificity/sensitivity information for individual kit components.

Source / Purification

Rabbit monoclonal antibodies are prepared by immunizing animals with a synthetic peptide corresponding to: the amino terminus of human β-tubulin, the sequence of human calnexin, residues surrounding Lys29 of human COX IV, the carboxy-terminal sequence of human histone H3 and human histone H2A, residues surrounding Pro671 of human NUP98, residues surrounding Thr298 of human fibrillarin, and residues near the amino terminus of LC3B. Mouse monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly190 of human Rab5A protein.

Background

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. One can confirm the subcellular location of a marker that colocalizes with one of the organelle-specific antibodies in this kit. While these antibodies serve as powerful tools for immunofluorescence, they may also be used as western blot controls for fractionated cell lysates.

Limited Uses

Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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