WB, IP
H M R
Endogenous
55
Rabbit IgG
#Q53EZ4
55165
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val459 of human CEP55 protein.
Background
The 55 kDa centrosomal protein (CEP55) is a widely expressed centrosome and midbody-associated protein that regulates cytokinesis, including completion of the final step during cytokinesis known as abscission (1,2). CEP55 activity during abscission is negatively regulated by p53 through Polo-like kinase 1 (3,4). The breast and ovarian cancer DNA repair protein BRCA2 interacts with CEP55 and plays a regulatory role during abscission (5). Research studies demonstrate that CEP55 is also involved in the regulation of Akt signaling, autophagy, and may be a biomarker in human cancer (reviewed in 6). The correlated overexpression of CEP55, the transcription factor FoxM1, and the HELLS helicase is seen in head and neck squamous cell carcinoma (7,8). Additional studies demonstrate that CEP55 expression regulates cell proliferation in gastric carcinoma (9).
- Fabbro, M. et al. (2005) Dev Cell 9, 477-88.
- Zhao, W.M. et al. (2006) Mol Biol Cell 17, 3881-96.
- Bastos, R.N. and Barr, F.A. (2010) J Cell Biol 191, 751-60.
- Chang, Y.C. et al. (2012) J Biol Chem 287, 4376-85.
- Mondal, G. et al. (2012) Dev Cell 23, 137-52.
- Jeffery, J. et al. (2015) Oncogene, [Epub ahead of print].
- Janus, J.R. et al. (2011) Laryngoscope 121, 2598-603.
- Waseem, A. et al. (2010) Oral Oncol 46, 536-42.
- Tao, J. et al. (2014) Tumour Biol 35, 4389-99.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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