WB, W-S
H
Endogenous
50
Rabbit IgG
#Q13231
1118
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser406 of human Chitotriosidase-1/CHIT1 protein.
Background
Chitin is an essential biopolymer structural component of many organisms, including arthropods, fungi, and parasites. Chitinases are part of the 18-glycoside hydrolase (18-GH) family of proteins that act to bind and degrade chitin. In mammals, this function provides defense against chitin-containing pathogens. Chitotriosidase-1, also known as Chitinase-1 or CHIT1, is the most abundant of these enzymes. CHIT1 consists of an N-terminal catalytic domain, a C-terminal chitin-binding domain, and a short hinge region that connects the two. CHIT1 activity is implicated in several inflammatory signaling pathways and is considered a sensitive biomarker for activated immune cells. It is produced and secreted by macrophages, microglia, and neutrophils in normal and inflammatory conditions. Elevated levels of CHIT1 in serum is a suggested biomarker for Gaucher's disease, atherosclerosis, pulmonary fibrosis, and some cancers. Elevated CSF levels of CHIT1 is also a suggested biomarker for amyotrophic lateral sclerosis (ALS) and other neurodegenerative diseases (1-5).
- Kanneganti, M. et al. (2012) J Epithel Biol Pharmacol 5, 1-9.
- Lee, C.M. et al. (2019) Life Sci Alliance 2, e201900350. doi: 10.26508/lsa.201900350.
- Majewski, S. et al. (2022) Front Immunol 13, 760776.
- Russo, C. et al. (2023) Int J Mol Sci 24, 6301. doi: 10.3390/ijms24076301.
- Mishra, P.S. et al. (2017) J Neuroinflammation 14, 251.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting W-S: Simple Western™
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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