Western blot analysis of extracts from various cell lines using HDAC4 (D8T3Q) Rabbit mAb.
Immunoprecipitation of HDAC5 from 293T cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is HDAC5 (D1J7V) Rabbit mAb. Western blot analysis was performed using HDAC5 (D1J7V) Rabbit mAb.
Western blot analysis of extracts from HeLa and Jurkat cells using HDAC6 (D21B10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HDAC7 (D4E1L) Rabbit mAb.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
Western blot analysis of extracts from various cell lines using HDAC5 (D1J7V) Rabbit mAb.
Western blot analysis of extracts from C2C12 cells, wild-type mouse embryonic fibroblasts (MEFs), and HDAC6 (-/-) MEFs using HDAC6 (D21B10) Rabbit mAb (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower).
Immunohistochemical analysis of paraffin-embedded K-562 (left) and LNCaP (right) cell pellets using HDAC7 (D4E1L) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using HDAC7 (D4E1L) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Immunohistochemical analysis of paraffin-embedded human tonsil using HDAC7 (D4E1L) Rabbit mAb.
Western blot analysis of extracts from various cell lines using HDAC7 (D4E1L) Rabbit mAb.
|HDAC4 (D8T3Q) Rabbit mAb 15164||20 µl||
||H Mk M R||140||Rabbit IgG|
|HDAC5 (D1J7V) Rabbit mAb 20458||20 µl||
||H Mk M R||140||Rabbit IgG|
|HDAC6 (D21B10) Rabbit mAb 7612||20 µl||
||H M R||160||Rabbit IgG|
|HDAC7 (D4E1L) Rabbit mAb 33418||20 µl||
||H Mk M||124||Rabbit IgG|
|Anti-rabbit IgG, HRP-linked Antibody 7074||100 µl||
The Class II HDAC Antibody Sampler Kit provides an economical means of detecting Class II HDAC proteins using control antibodies against HDAC4, HDAC5, HDAC6, and HDAC7. The kit contains enough primary antibodies to perform at least two western blot experiments.
Each antibody in the Class II HDAC Antibody Sampler Kit detects endogenous levels of its target protein. Cross-reactivity was not observed with other family members.
Monoclonal antibodies are produced by immunizing rabbits with synthetic peptides corresponding to the amino terminus of human HDAC4, Leu445 of human HDAC5, the carboxy terminus of HDAC6, and Leu66 of HDAC7 proteins.
Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).
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