WB, IP, ChIP, ChIP-seq
H M R Mk
Endogenous
65
Rabbit IgG
#Q8WX92
25920
Product Information
Product Usage Information
For optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 106 cells) per IP. This antibody has been validated using SimpleChIP® Enzymatic Chromatin IP Kits.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Chromatin IP | 1:50 |
| Chromatin IP-seq | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
Hamster, Bovine
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COBRA1 protein.
Background
Negative Elongation Factor (NELF) consists of four subunits: WHSC2 (NELF-A), COBRA-1 (NELF-B), TH1L (NELF-C/D), and NELF-E (1). NELF, together with DRB-sensitivity inducing factor (DSIF), inhibits RNA Polymerase II (RNAPII) elongation resulting in RNAPII promoter proximal pausing, where it waits additional signaling to resume transcription (2,3). The release of RNAPII from promoter proximal pausing is a critical regulatory point during transcription and is signaled by positive transcription elongation factor (p-TEF-b) phosphorylation of both NELF and the carboxy-terminal domain (CTD) within the largest subunit of RNAPII (3,4). WHSC2 is thought to connect the NELF complex to RNAPII machinery, while NELF-E contains an RNA binding motif that is necessary for NELF function (1,5,6). TH1L, together with COBRA-1, are integral subunits that bring WHSC2 and NELF-E together in the NELF complex (1).
Cofactor of BRCA1 (COBRA1, NELF-B) was first identified as a factor that interacts with the BRCT domain of the tumor suppressor protein BRCA1 (7). COBRA1 is a modulator of ligand dependent and independent expression of estrogen receptor-α target genes (8,9). COBRA1 expression is reduced in metastatic and recurrent breast cancer, suggesting that low levels of COBRA1 in breast cancers may serve as an indicator for poor prognosis (10).
- Narita, T. et al. (2003) Mol Cell Biol 23, 1863-73.
- Nechaev, S. and Adelman, K. (2011) Biochim Biophys Acta 1809, 34-45.
- Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
- Buratowski, S. (2009) Mol Cell 36, 541-6.
- Yamaguchi, Y. et al. (2001) Science 293, 124-7.
- Yamaguchi, Y. et al. (2002) Mol Cell Biol 22, 2918-27.
- Ye, Q. et al. (2001) J Cell Biol 155, 911-21.
- Aiyar, S.E. et al. (2004) Genes Dev 18, 2134-46.
- Aiyar, S.E. et al. (2007) Oncogene 26, 2543-53.
- Sun, J. et al. (2008) J Cell Biochem 103, 1798-807.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation ChIP: Chromatin IP ChIP-seq: Chromatin IP-seq
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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