WB
H
Endogenous
165
Rabbit
#P08123
1278
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn507 of human COL1A2 protein. Antibodies are purified by peptide affinity chromatography.
Background
Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex comprised of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The loss-of-function mutations in the COL1A1 and COL1A2 genes are a major causative factor for osteogenesis imperfecta (brittle bone disease) (4). Notably, COL1A2 expression levels have also been associated with tumor development in gastric cancer, pancreatic cancer, and chondrosarcoma (5-7). Research studies suggest that upregulation of COL1A2 can generate a modified extracellular matrix environment that promotes cancer cell proliferation, migration, and invasion.
- Prockop, D.J. and Kivirikko, K.I. (1995) Annu Rev Biochem 64, 403-34.
- Chang, S.W. et al. (2012) Biophys J 102, 640-8.
- Zhang, Y. and Stefanovic, B. (2016) Int J Mol Sci 17, 419.
- Forlino, A. et al. (2011) Nat Rev Endocrinol 7, 540-57.
- Pan, H. et al. (2021) Cancer Cell Int 21, 7.
- Wu, J. et al. (2019) J Enzyme Inhib Med Chem 34, 420-428.
- Omar, R. et al. (2019) Cancer Lett 459, 227-239.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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