WB
H
Endogenous
200
Rabbit IgG
#P02458
1280
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala194 of human COL2A1 protein.
Background
COL2A1 (α1 type II collagen) is a fibril-forming collagen encoded by the gene COL2A1 (1,2). The protein forms homotrimeric type II collagen and is the main extracellular component in hyaline cartilage extracellular matrix (ECM), nucleus pulposus of intervertebral discus, vitreous humor of the eye, and inner ear structure (3). Type II collagen plays a fundamental role in maintaining cartilage formation, differentiation, and growth (4-6). Mutations of COL2A1 cause impairment of ECM deposition and signaling, which contribute to various forms of tissue dysplasia and pathologies such as Stickler syndrome and chondrosarcoma (3,7,8). At the ECM, COL2A1 interacts with integrins such as integrin β1 and growth factors such as IL1β, IL6, IL8, and others, which activate intracellular signaling pathways, including SMAD1, and MAP kinases p38, JNK, and ERK (9-12).
- Cheah, K.S. et al. (1985) Proc Natl Acad Sci USA 82, 2555-9.
- Ricard-Blum, S. (2011) Cold Spring Harb Perspect Biol 3, a004978.
- Deng, H. et al. (2016) Mutat Res Rev Mutat Res 768, 1-13.
- Bosnakovski, D. et al. (2006) Biotechnol Bioeng 93, 1152-63.
- Barbieri, O. et al. (2003) Am J Physiol Cell Physiol 285, C1504-12.
- Gauci, S.J. et al. (2019) Matrix Biol 83, 77-96.
- Nenna, R. et al. (2019) Appl Clin Genet 12, 235-238.
- Tarpey, P.S. et al. (2013) Nat Genet 45, 923-6.
- Klatt, A.R. et al. (2009) J Pathol 218, 241-7.
- Lian, C. et al. (2017) Mol Med Rep 16, 4730-4736.
- Klatt, A.R. et al. (2009) J Orthop Res 27, 65-70.
- Lian, C. et al. (2019) Bone Res 7, 8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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