WB
H
Endogenous
200
Rabbit
#P02462
1282
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp536 of human COL4A1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Type IV collagens support extracellular matrix (ECM) function by forming a cross-linking network in the basement membrane zone. There are six different type IV collagens that are encoded by the following genes: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5, and COL4A6. Among them, COL4A1 and COL4A2 are the most common collagens found in the basement membrane, and are usually used as a marker of the basement membrane dynamic stability (1). The type IV collagen fiber exists as a trimeric complex composed of two molecules of COL4A1 and one molecule of COL4A2. The N-terminal 7S domains of the protein function on intermolecular cross-linking, and C-terminal non-collagenous domains (NCI) are responsible for heterotrimer assembly. The trimeric fiber further associates to form a cross-linked sheet structure by NCI/NCI and 7S/7S interaction (2). COL4A1 plays a critical role in maintaining basement membrane integrity for normal tissue functions. Genetic mutations of COL4A1 and COL4A2 have been linked to cardiovascular disease, cerebral small vessel disease, and glomerulocystic kidney disease (3-5).
- Jeanne, M. and Gould, D.B. (2017) Matrix Biol 57-8, 29-44.
- Kuo, D.S. et al. (2012) Hum Mol Genet 21, R97-110.
- Steffensen, L.B. and Rasmussen, L.M. (2018) Am J Physiol Heart Circ Physiol 315, H610-H625.
- Giau, V.V. et al. (2019) Int J Mol Sci 20, pii: E4298. doi: 10.3390/ijms20174298.
- Chen, Z. et al. (2016) J Am Soc Nephrol 27, 1042-54.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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