Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with siRNA targeting human COPS6 (+), using COPS6 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from various cell lines using COPS6 Antibody.
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Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
COPS6 Antibody recognizes endogenous levels of total COPS6 protein. This antibody also cross-reacts with an unidentifed protein of 78 kDa protein in some cell extracts.
Human, Mouse, Rat
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COPS6 protein. Antibodies are purified by protein A and peptide affinity chromatography.
COP9 signalosome complex subunit 6 (COPS6) belongs to the multisubunit COP9 signalsome which modulates protein ubiquitination to affect cellular processes such as cell-cycle progression and DNA damage repair (1). COPS6 is a putative oncogene that has been shown to be overexpressed in a significant percentage of colorectal cancers with poor prognosis (2). Mechanistically, research studies have shown that a signaling axis involving EGFR and ERK promotes tumor growth through the phosphorylation of COPS6, which indirectly facilitates downstream stabilization of β-catenin and enhancement of its transcriptional acitivity (2). Reasearch studies have also demonstrated that COPS6 contributes to tumorigenesis through the stabilization of other oncoproteins like c-Myc, MDM2 and E6AP (3-5).
Explore pathways + proteins related to this product.
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