WB
H
Endogenous
65
Rabbit IgG
#Q9UKL0
23186
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CoREST protein.
Background
The REST corepressor 1 (CoREST, RCOR1) was first identified as a repressor element 1-silencing transcription factor (REST) corepressor (1,2). The CoREST protein is encoded by the RCOR1 gene and is part of a large, multi-subunit repressor complex that includes the histone demethylase LSD1 and histone deacetylases (HDAC) 1 and 2 (1,3-5). CoREST binds the carboxy-terminal domain of REST and is recruited to repress neuronal gene transcription in non-neuronal and neural stem cells (1,6,7). The REST corepressor is essential for repressor complex-nucleosome interaction, the subsequent deacetylation of histone amino-terminal tails by HDAC1/2, and the LSD1 methylation of histone H3 at Lys4 (8-10). The targeting of CoREST to genes that are not repressed by REST suggests a role apart from neural cell fate regulation. These include growth factor independent (Gfi) target genes during erythroid differentiation, targets of carboxy-terminal binding protein (CtBP), and heat shock and pro-inflammatory response genes (11-15).
- Qureshi, I.A. et al. (2010) Cell Cycle 9, 4477-86.
- Barrios, Á.P. et al. (2014) Mol Cell Biol 34, 2760-70.
- Andrés, M.E. et al. (1999) Proc Natl Acad Sci U S A 96, 9873-8.
- Humphrey, G.W. et al. (2001) J Biol Chem 276, 6817-24.
- You, A. et al. (2001) Proc Natl Acad Sci U S A 98, 1454-8.
- Ballas, N. et al. (2005) Cell 121, 645-57.
- Ballas, N. and Mandel, G. (2005) Curr Opin Neurobiol 15, 500-6.
- Lee, M.G. et al. (2005) Nature 437, 432-5.
- Shi, Y.J. et al. (2005) Mol Cell 19, 857-64.
- Yang, M. et al. (2006) Mol Cell 23, 377-87.
- Saleque, S. et al. (2007) Mol Cell 27, 562-72.
- Yao, H. et al. (2014) Blood 123, 3175-84.
- Gómez, A.V. et al. (2008) Mol Cell 31, 222-31.
- Saijo, K. et al. (2009) Cell 137, 47-59.
- Chinnadurai, G. (2002) Mol Cell 9, 213-24.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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