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#12140Store at -20C

Cell Signaling Technology

Orders: 877-616-CELL (2355) [email protected]

Support: 877-678-TECH (8324)

Web: [email protected] cellsignal.com

3 Trask LaneDanversMassachusetts01923USA
For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:

WB

REACTIVITY:

H Mk

SENSITIVITY:

Endogenous

MW (kDa):

130

SOURCE:

Rabbit

UniProt ID:

#O43303

Entrez-Gene Id:

9738

Product Information

Product Usage Information

Application Dilution
Western Blotting 1:1000

Storage

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

Specificity / Sensitivity

CP110 Antibody recognizes endogenous levels of total CP110 protein. This antibody cross-reacts with a protein of unknown origin at ~65 kDa.

Species Reactivity:

Human, Monkey

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues around Ser580 of human CP110 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The centrosome is an organelle that plays an important role in the mammalian cell cycle. Prior to the onset of mitosis, the single interphase centrosome duplicates only once, creating a pair of daughter centrosomes that will form the two spindle poles after breakdown of the nuclear envelope. Failure to duplicate or overduplication of the centrosome can result in polyploidy and genomic instability (reviewed in 1). Centrosomal protein of 110 kDa (CP110) is a cyclin-dependent kinase (CDK) substrate that plays a critical role in promoting the duplication of centrosomes and correct spindle formation (2). In addition, CP110 has been shown to interact with calmodulin (CaM) and centrin to regulate the progression through cytokinesis (3), and with Cep97 and Cep290 to regulate the formation of primary cilia (4,5). CP110 expression is induced in G1/S with peak expression during S-phase. Degradation of CP110 is mediated by cyclin F in G2-phase and is required for normal progression into M-phase (6).

  1. Hinchcliffe, E.H. and Sluder, G. (2001) Genes Dev 15, 1167-81.
  2. Chen, Z. et al. (2002) Dev Cell 3, 339-50.
  3. Tsang, W.Y. et al. (2006) Mol Biol Cell 17, 3423-34.
  4. Spektor, A. et al. (2007) Cell 130, 678-90.
  5. Tsang, W.Y. et al. (2008) Dev Cell 15, 187-97.
  6. D'Angiolella, V. et al. (2010) Nature 466, 138-42.

Species Reactivity

Species reactivity is determined by testing in at least one approved application (e.g., western blot).

Western Blot Buffer

IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

Applications Key

WB: Western Blotting

Cross-Reactivity Key

H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected

Trademarks and Patents

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit cellsignal.com/trademarks for more information.

Limited Uses

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