Western blot analysis of extracts from various cell lines using CR2/CD21 Antibody (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). As expected, CR2/CD21 protein expression is not detected in K-562 cells.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CD21 protein (hCD21-Myc/DDK; +), using CR2/CD21 Antibody (upper), DYKDDDDK Tag Antibody #2368 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
NOTE: Please refer to primary antibody product webpage for recommended antibody dilution.
From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: #12957 Western Blotting Application Solutions Kit
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
Load 20 µl onto SDS-PAGE gel (10 cm x 10 cm).
NOTE: Volumes are for 10 cm x 10 cm (100 cm2) of membrane; for different sized membranes, adjust volumes accordingly.
* Avoid repeated exposure to skin.
posted June 2005
revised June 2020
Protocol Id: 10
CR2/CD21 Antibody recognizes endogenous levels of total CR2/CD21 protein.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp430 of human CR2/CD21 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Complement receptor type 2 (CR2/CD21) is a type 1 transmembrane glycoprotein whose expression is largely restricted to B lymphocytes and follicular dendritic cells (1,2). Research studies have shown that CR2/CD21 functions to bind the complement fragments iC3b, C3dg, and C3d, which function to activate the alternative complement pathway and MAC formation (3,4). In addition to its function as a complement receptor, CR2/CD21 also functions as the B-lymphocyte receptor for Epstein-Barr virus (5) and interferon alpha (6). Research studies have also shown that CR2/CD21 participates in B-cell activation, proliferation, and protection from apoptosis through its association with components of the B-cell coreceptor signaling complex such as CD19 and CD21 (7-9).
Explore pathways + proteins related to this product.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Tween is a registered trademark of ICI Americas, Inc.