WB, IHC-Bond, IHC-P
H
Endogenous
25-80
Rabbit IgG
#Q9NQ25
57823
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:400 |
| Immunohistochemistry (Paraffin) | 1:150 |
Storage
For a carrier free (BSA and azide free) version of this product see product #66110.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human CRACC/SLAMF7/CD319 protein.
Background
CRACC/SLAMF7/CD319 (also known as CS1) is a member of the signaling lymphocytic activation molecule (SLAM) family. It is a single-pass type l transmembrane glycoprotein expressed on NK cells, subsets of mature dendritic cells, activated B and T lymphocytes, but not in promyelocytic B or T cell lines. Expression of this protein has been detected in the spleen, lymph node, peripheral blood leukocytes, bone marrow, small intestine, stomach, appendix, lung, and trachea (1-5). Homophilic interactions of CRACC/SLAMF7/CD319 modulate the activity and differentiation of immune cells. CRACC/SLAMF7/CD319 may function as an inhibitory or activating receptor in immune cells depending on cellular context and availability of adapter proteins, SH2D1A/SAP and/or SH2D1B/EAT-2 (4-8). In the presence of SH2D1B/EAT-2, CRACC/SLAMF7/CD319 activates NK cells and B cells (4-6). T cells lack SH2D1B/EAT-2 expression, and therefore CRACC/SLAMF7/CD319 acts as an inhibitory receptor (7). In LPS-activated monocytes, CRACC/SLAMF7/CD319 negatively regulates production of proinflammatory cytokines (8). CRACC/SLAMF7/CD319 is upregulated in multiple myeloma and is implicated in the uncontrolled proliferation of these cells, and thus has become the target for therapeutic intervention (9,10). Seven isoforms of CRACC/SLAMF7/CD319 produced by alternative splicing have been identified.
- Boles, K.S. and Mathew, P.A. (2001) Immunogenetics 52, 302-7.
- Tovar, V. et al. (2002) Immunogenetics 54, 394-402.
- Murphy, J.J. et al. (2002) Biochem J 361, 431-6.
- Bouchon, A. et al. (2001) J Immunol 167, 5517-21.
- Kumaresan, P.R. et al. (2002) Mol Immunol 39, 1-8.
- Lee, J.K. et al. (2007) J Immunol 179, 4672-8.
- Cruz-Munoz, M.E. et al. (2009) Nat Immunol 10, 297-305.
- Kim, J.R. et al. (2013) Inflamm Res 62, 765-72.
- Tai, Y.T. et al. (2008) Blood 112, 1329-37.
- Hsi, E.D. et al. (2008) Clin Cancer Res 14, 2775-84.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IHC-Bond: IHC Leica Bond IHC-P: Immunohistochemistry (Paraffin)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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