WB, IP
H M R Mk
Endogenous
75
Rabbit IgG
#Q68CJ9
84699
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu93 of human CREB-H protein.
Background
CREB-H belongs to the bZIP transmembrane transcription factor family that activates transcription by binding to cAMP responsive elements (1,2). CREB-H interacts with ATF-6 and binds to conserved elements in the APR genes to synergistically activate transcription (2-4). Evidence suggests that CREB-H is activated by cleavage upon ER stress, inflammatory stimuli (2-5), and metabolic stress (5,6). Known chemical activators of ER stress, such as tunicamycin and thapsigargin, have been shown to induce cleavage of the full-length 75 kDa from of CREB-H, releasing the 50 kDa N-terminal fragment, which translocates to the nucleus (1-4). Upon ER stress, the transmembrane domain of CREB-H is cleaved by Golgi proteases, which allows subsequent translocation to the nucleus. Liberated nuclear CREB-H plays a crucial role in the acute systemic inflammatory response by activating transcription of genes that encode serum amyloid P-component (SAP) and C-reactive protein (CRP) (2,3). Recent studies suggest that activated CREB-H functions as a crucial metabolic regulator of hepatic lipogenesis, fatty acid (FA) oxidation, and lipolysis (5,6). Metabolic stress inducers, such as saturated fatty acids, insulin, and atherogenic high-fat diets have been shown to activate CREB-H in the liver (5-7).
- Omori, Y. et al. (2001) Nucleic Acids Res 29, 2154-62.
- Chin, K.T. et al. (2005) Nucleic Acids Res 33, 1859-73.
- Zhang, K. et al. (2006) Cell 124, 587-99.
- DeBose-Boyd, R.A. et al. (1999) Cell 99, 703-12.
- Zhang, C. et al. (2012) Hepatology 55, 1070-82.
- Lee, A.H. (2012) Curr Opin Lipidol 23, 141-6.
- Lee, J.H. et al. (2011) Nat Med 17, 812-5.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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