WB, IP
H M R
Endogenous
22
Rabbit IgG
#P02511
1410
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu165 of human CRYAB protein.
Background
CRYAB (αB-Crystallin) is a member of the small heat shock protein (sHSP also known as HSP20) family (1). This protein was initially found to be overexpressed in the eye lens, and later also detected at high levels in heart and skeletal muscle tissues (2,3). CRYAB functions mainly as a molecular chaperone, responding to stress by binding unfolded target proteins to prevent aggregation (4,5). Research studies have shown that elevated expression of CRYAB in neurological disease and stroke patients protects tissue and cells from damage under extreme stress, leading to the investigation of CRYAB as a potential therapeutic target (6-9). Researchers also found that expression of the missense mutation of CRYAB (R120G) in the mouse model causes cardiomyopathy due to abnormal desmin aggregation (10). At the molecular level, CRYAB is involved in multiple biological processes, such as inhibiting apoptosis by binding and inhibiting caspase and proapoptotic Bax and Bcl-xS protein functions (11,12), promoting angiogenesis by binding and stabilizing VEGF for secretion (13), and regulating cytoskeletal organization through association with actin filament, intermediate filament, and cardiac titin (14-16).
- Mehlen, P. et al. (1996) EMBO J 15, 2695-706.
- Piatigorsky, J. (1989) FASEB J 3, 1933-40.
- Bennardini, F. et al. (1992) Circ Res 71, 288-94.
- Benjamin, I.J. and McMillan, D.R. (1998) Circ Res 83, 117-32.
- Horwitz, J. (1992) Proc Natl Acad Sci U S A 89, 10449-53.
- Ousman, S.S. et al. (2007) Nature 448, 474-9.
- Ray, P.S. et al. (2001) FASEB J 15, 393-402.
- Arac, A. et al. (2011) Proc Natl Acad Sci U S A 108, 13287-92.
- Sanbe, A. (2011) Biol Pharm Bull 34, 1653-8.
- Wang, X. et al. (2001) Circ Res 89, 84-91.
- Kamradt, M.C. et al. (2001) J Biol Chem 276, 16059-63.
- Mao, Y.W. et al. (2004) Cell Death Differ 11, 512-26.
- Kase, S. et al. (2010) Blood 115, 3398-406.
- Wang, K. and Spector, A. (1996) Eur J Biochem 242, 56-66.
- Nicholl, I.D. and Quinlan, R.A. (1994) EMBO J 13, 945-53.
- Zhu, Y. et al. (2009) J Biol Chem 284, 13914-23.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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