Immunohistochemical analysis of paraffin-embedded Daudi cell pellets (left, positive) and Jurkat cell pellets (right, negative) using CXCR5 (D6L3C) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CXCR5 (D6L3C) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma using CXCR5 (D6L3C) Rabbit mAb (IHC Specific).
Immunohistochemical analysis of paraffin-embedded human tonsil using CXCR5 (D6L3C) Rabbit mAb (IHC Specific).
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
NOTE: Prepare solutions with reverse osmosis deionized (RODI) or equivalent grade water.
NOTE: Do not allow slides to dry at any time during this procedure.
For Citrate: Heat slides in a microwave submersed in 1X citrate unmasking solution until boiling is initiated; follow with 10 min at a sub-boiling temperature (95°-98°C). Cool slides on bench top for 30 min.
|SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114||SignalStain® Boost IHC Detection Reagent (AP, Rabbit) #18653|
|SignalStain® DAB Substrate Kit #8059||SignalStain® Vibrant Red Alkaline Phosphatase Substrate Kit #76713|
|SignalStain® Vivid Purple Peroxidase Substrate Kit #96632|
NOTE: Use of detection reagents other than those specified in this protocol may require further optimization of the primary antibody to account for the different sensitivities of the detection reagents.
posted February 2010
revised June 2020
Protocol Id: 283
CXCR5 (D6L3C) Rabbit mAb (IHC Specific) recognizes endogenous levels of total CXCR5 protein.
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of human CXCR5 protein.
CXCR5 is a G protein-coupled receptor belonging to the chemokine receptor subfamily (1). Upon binding of its ligand, the chemokine CXCL13, CXCR5 initiates multiple intracellular signaling pathways that regulate cell proliferation, survival, and migration (2). CXCR5 is expressed in both mature B cells and follicular helper T cells, and respond to CXCL13 gradient to control lymphocyte migration towards secondary lymphoid tissues (3). CXCR5 has also been shown to be highly expressed in primary breast tumors, in correlation with their propensity to grow and metastasize (4).
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