| Product Includes | Product # | Quantity | Mol. Wt | Isotype/Source |
|---|---|---|---|---|
| p15 INK4B Antibody | 4822 | 40 µl | 15 kDa | Rabbit |
| p27 Kip1 (SX53G8.5) Mouse mAb | 3698 | 40 µl | 27 kDa | Mouse IgG1 |
| p18 INK4C (DCS118) Mouse mAb | 2896 | 40 µl | 18 kDa | Mouse IgG2a |
| p21 Waf1/Cip1 (12D1) Rabbit mAb | 2947 | 40 µl | 21 kDa | Rabbit IgG |
| p57 Kip2 Antibody | 2557 | 40 µl | 57 kDa | Rabbit |
| p27 Kip1 (D69C12) XP® Rabbit mAb | 3686 | 40 µl | 27 kDa | Rabbit IgG |
| Anti-rabbit IgG, HRP-linked Antibody | 7074 | 100 µl | Goat | |
| Anti-mouse IgG, HRP-linked Antibody | 7076 | 100 µl | Horse |
Please visit cellsignal.com for individual component applications, species cross-reactivity, dilutions, protocols, and additional product information.
Description
The Cyclin Dependent Kinase Inhibitor Antibody Sampler Kit provides an economical means to investigate cyclin dependent kinase inhibitors. The kit contains enough primary and secondary antibody to perform four western blot experiments.
Storage
Background
The cyclin-dependent kinase complex can exist as an active, binary complex containing cyclin and kinase or as an inactive, ternary complex when the pair associates with a CKI inhibitor. Some of these component proteins act selectively within their cyclin/CDK complex while other proteins exhibit a broader range of functions, including roles apart from the CDK complex (1,2). The INK4 family is characterized by 32 amino acid ankyrin repeats that mediate specific interactions with CDK4/6 and cyclinD/CDK4/6 (3). The Kip family includes p27, perhaps one of the most versatile proteins by virtue of its inherently context-adaptive structure. p27 has been implicated in regulatory roles of cell cycle progression, malignant transformation, cell motility, and differentiation (4,5). Similarly, p21 interacts with several CDK complexes during different cell cycle stages to exert different effects; p21 is regulated by phosphorylation and ubiquitin-mediated degradation (6,7).
- Harper, J.W. (1997) Cancer Surv 29, 91-107.
- Viallard, J.F. et al. (2001) Cancer Radiother 5, 109-29.
- Noh, S.J. et al. (1999) Cancer Res 59, 558-64.
- Borriello, A. et al. (2007) Cell Cycle 6, 1053-61.
- Chassot, A.A. et al. (2008) Cell Cycle 7, 2038-46.
- Kim, Y. et al. (2008) Genes Dev 22, 2507-19.
- Abbas, T. and Dutta, A. (2009) Nat Rev Cancer 9, 400-14.
Background References
Trademarks and Patents
Limited Uses
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