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Cystatin F/CST7 (F6Y9Q) Rabbit mAb #71093

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  • WB

    Product Specifications

    REACTIVITY H M
    SENSITIVITY Endogenous
    MW (kDa) 16-25
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    Species Cross-Reactivity Key:
    • H-Human 
    • M-Mouse 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:10 - 1:50

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    Cystatin F/CST7 (F6Y9Q) Rabbit mAb recognizes endogenous levels of total cystatin F/CST7 protein.

    Species Reactivity:

    Human, Mouse

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro35 of mouse cystatin F/CST7 protein.

    Background

    Cystatin F, also known as CST7, is an endosomal-lysosomal cysteine protease inhibitor member of the cystatin type 2 superfamily, encoded by the CST7 gene (1). It is selectively expressed by hemopoietic cells such as cytotoxic T cells, natural killer cells, monocytes, and dendritic cells, suggesting a role in immune regulation (1,2). CST7 is expressed as an inactive dimer and converted to a functionally active monomer localized to the endo-lysosomal compartment (3). CST7 has been implicated in various cancer types, including breast and cervical cancer, and is thought to play a role in modulating the tumor microenvironment (4,5). Additionally, CST7 is highly upregulated in disease-associated microglia in Alzheimer’s disease and other neurodegenerative diseases, and may play a sex-specific role in neurodegeneration (6,7).
    For Research Use Only. Not for Use in Diagnostic Procedures.
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    KARPAS cell line source: Dr. Abraham Karpas at the University of Cambridge.
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