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9384
Cytokeratin Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cytokeratin Antibody Sampler Kit #9384

Citations (2)
Western blot analysis of extracts from HeLa and MCF7 cells using Keratin 7 (D1E4) XP® Rabbit mAb. Note that MCF7 cells are negative for Keratin 7, as expected.
Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17.
Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.
Western blot analysis of extracts from various cell lines, using Keratin 8/18 (C51) Mouse mAb.
Western blot analysis of extracts from HeLa and A431 cells, using Keratin 18 (DC10) Mouse mAb.
Western blot analysis of extracts from various cell types using Keratin 19 (BA17) Mouse mAb. As expected, the protein is absent in A-431 and HeLa cells.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.
After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.
Confocal immunofluorescent analysis of HeLa (left) and MCF7 (right) cells using Keratin 7 (D1E4) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Keratin 8/18 (C51) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic localization using Keratin 18 (DC10) Mouse mAb.
Immunhistochemical analysis of paraffin-embedded human breast carcinoma using Keratin 19 (BA17) Mouse mAb.
Flow cytometric analysis of Jurkat cells (blue) and HeLa cells (green) using Keratin 7 (D1E4) XP® Rabbit mAb (solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Keratin 8/18 (C51) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using Keratin 18 (DC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded MCF-7 cells (positive, left) and A-431 cells (negative, right), using Keratin 19 (BA17) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the lung, using Keratin 8/18 (C51) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human benign prostate hyperplasia, using Keratin 18 (DC10) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Keratin 8/18 (C51) Mouse mAb.
Confocal immunofluorescent analysis of HepG2 (left) and SH-SY5Y (right) cells using Keratin 18 (DC10) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Keratin 8/18 (C51) Mouse mAb.
Flow cytometric analysis of Jurkat cells (blue) and HT-29 cells (green) using Keratin 18 (DC10) Mouse mAb (solid lines) or a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb.
Confocal immunofluorescent analysis of HeLa cells using Keratin 8/18 (C51) Mouse mAb (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087.
Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb.
Flow cytometric analysis of MCF-7 cells, using Keratin 8/18 (C51) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb.
Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of A-431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
To Purchase # 9384
Cat. # Size Qty. Price
9384T
1 Kit  (6 x 20 microliters)

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Keratin 17 (D73C7) Rabbit mAb 4543 20 µl
  • WB
  • IF
  • F
H M R 48 Rabbit IgG
Keratin 8/18 (C51) Mouse mAb 4546 20 µl
  • WB
  • IHC
  • IF
  • F
H Mk 46 Keratin 18. 55 Keratin 8. Mouse IgG1
Keratin 18 (DC10) Mouse mAb 4548 20 µl
  • WB
  • IHC
  • IF
  • F
H 46 Mouse IgG1
Keratin 19 (BA17) Mouse mAb 4558 20 µl
  • WB
  • IHC
H 40 Mouse IgG1
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
Keratin 7 (D1E4) XP® Rabbit mAb 4465 20 µl
  • WB
  • IF
  • F
H 52 Rabbit IgG
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 

Product Description

The Cytokeratin Antibody Sampler Kit provides an economical means to evaluate the presence and status of selected keratin proteins. The kit provides enough primary and secondary antibodies to perform two Western blot experiments per primary antibody.

Specificity / Sensitivity

The pan-keratin (C11) mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13 and 18. The antibody does not cross-react with other keratins. Each of the remaining antibodies included in this kit detect endogenous levels of the specified keratin protein and do not cross-react with other keratin proteins.

Source / Purification

Pan-Keratin Mouse mAb (C11) is produced by immunizing animals with a cytoskeleton preparation from A431 cells. Keratin 8/18 (C51) Mouse mAb is produced by immunizing animals with a cytoskeleton preparation from HeLa cells. Keratin 18 (DC10) Mouse mAb is produced by immunizing animals with human PMC-42 breast carcinoma cells. Keratin 19 (BA17) Mouse mAb is produced by immunizing animals with detergent-insoluble extract of human mammary epithelial organoids. Keratin 7 (D1E4) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 7 protein and Keratin 17 (D73C7) XP® Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins K9-K28) and a basic keratin (or type II keratin, keratins K1-K8 and K71-K80) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research and clinical biomarkers (1,2).

Dysregulation/mutations in keratin genes can lead to a variety of disorders affecting the skin, hair, nails, and other epithelial tissues (3). While expression of keratins can be variable, immunohistochemical staining of keratins is widely used to help in the identification and classification of epithelial tumors, and may also provide prognostic information.

Keratins 8 and 18 (K8/K18) are expressed in simple epithelia of normal tissue, as well as in adenocarcinomas of the breast, lung, ovary, and gastrointestinal tract. Keratin 17 is expressed in basal keratinocytes of stratified epithelia, hair follicles, and sebaceous glands. Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development (4). Keratin 14 (K14) is expressed in basal cells of stratified epithelia, and in basal-like subtypes of breast cancer and squamous cell carcinomas. Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas. Keratin 5/6 (K5/6) is expressed in basal cells of stratified epithelia, including the skin, prostate, and breast, as well as in basal-like breast cancers, squamous cell carcinomas, and some lung carcinomas. Keratin 7 (K7) is expressed in glandular epithelia, such as those in the lung, breast, and female reproductive tract, as well as in adenocarcinomas of the lung, breast, and ovary (5,6).

Keratins, particularly K8, K18, and K19, serve as biomarkers for identification of circulating tumor cells (CTCs) (5).

Post-translational modifications, including phosphorylation, acetylation, ubiquitylation, sumoylation, glycosylation, and transamidation, have been shown to affect the functions of keratins in normal and disease states (6). Understanding the molecular mechanisms underlying these PTMs may provide insights into cancer pathogenesis.

Limited Uses

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For Research Use Only. Not for Use in Diagnostic Procedures.
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