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8614
Cytoskeletal Marker Antibody Sampler Kit
Primary Antibodies
Antibody Sampler Kit

Cytoskeletal Marker Antibody Sampler Kit #8614

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Cytoskeletal Marker Antibody Sampler Kit: Image 1

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb #8457.

Cytoskeletal Marker Antibody Sampler Kit: Image 2

Confocal immunofluorescent analysis of mouse skeletal muscle (left, positive) or liver (right, negative), using Desmin (D93F5) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Cytoskeletal Marker Antibody Sampler Kit: Image 3

Flow cytometric analysis of A549 cells (red) and HeLa cells (blue) using Keratin 17 (D73C7) Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 4

Flow cytometric analysis of A431 cells using Pan-Keratin (C11) Mouse mAb (solid line) versus a concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed line). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Cytoskeletal Marker Antibody Sampler Kit: Image 5

Flow cytometric analysis of NIH/3T3 cells using β-Tubulin (9F3) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

Cytoskeletal Marker Antibody Sampler Kit: Image 6

Flow cytometric analysis of MCF7 cells (blue) and HeLa cells (green) using Vimentin (D21H3) XP® Rabbit mAb(solid lines) or a concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Cytoskeletal Marker Antibody Sampler Kit: Image 7

Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytoskeletal Marker Antibody Sampler Kit: Image 8

Flow cytometric analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Cytoskeletal Marker Antibody Sampler Kit: Image 9

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO® is added and emits light during enzyme catalyzed decomposition.

Cytoskeletal Marker Antibody Sampler Kit: Image 10

Confocal immunofluorescent analysis of C2C12 cells (left, positive) or Neuro-2a cells (right, negative), using Desmin (D93F5) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Cytoskeletal Marker Antibody Sampler Kit: Image 11

Confocal immunofluorescent analysis of HeLa (left) and A549 (right) cells using Keratin 17 (D73C7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytoskeletal Marker Antibody Sampler Kit: Image 12

Confocal immunofluorescent analysis of HeLa cells using Pan-Keratin (C11) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

Cytoskeletal Marker Antibody Sampler Kit: Image 13

Confocal immunofluorescent analysis of HeLa cells using β-Tubulin (9F3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytoskeletal Marker Antibody Sampler Kit: Image 14

Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

Cytoskeletal Marker Antibody Sampler Kit: Image 15

Confocal immunofluorescent analysis of HeLa cells using β-Actin (D6A8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Cytoskeletal Marker Antibody Sampler Kit: Image 16

Western blot analysis of extracts from C2C12 cells, rat heart and human heart using Desmin (D93F5) XP® Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 17

Western blot analysis of extracts from A-431, HeLa, and A549 cells using Keratin 17 (D73C7) Rabbit mAb. As expected, A549 cells are negative for keratin 17.

Cytoskeletal Marker Antibody Sampler Kit: Image 18

Immunohistochemical analysis of paraffin-embedded human transitional epithelial carcinoma (bladder), using Pan-Keratin (C11) Mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 19

Immunohistochemical analysis of paraffin-embedded human glioblastoma using β-Tubulin (9F3) Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 20

Western blot analysis of extracts from various cell lines using β-Actin (D6A8) Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 21

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Pan-Keratin (C11) Mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 22

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Tubulin (9F3) Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 23

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vimentin (D21H3) XP® Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 24

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Pan-Keratin (C11) Mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 25

Immunohistochemical analysis of paraffin-embedded human melanoma using β-Tubulin (9F3) Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 26

Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Cytoskeletal Marker Antibody Sampler Kit: Image 27

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using Pan-Keratin (C11) Mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 28

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Tubulin (9F3) Rabbit mAb preincubated with control peptide (left) or β-Tubulin Blocking Peptide #1032 (right).

Cytoskeletal Marker Antibody Sampler Kit: Image 29

Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 30

Immunohistochemical analysis of paraffin-embedded H358 xenograft, using Pan-Keratin (C11) Mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 31

Western blot analysis of extracts from COS-7, NIH/3T3 and PC12 cells, using β-Tubulin (9F3) Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 32

Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 33

Immunohistochemical analysis of paraffin-embedded human ductal breast carcinoma using Pan-Keratin (C11) mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 34

Western blot analysis of extracts from control HeLa cells (lane 1) or Vimentin knockout HeLa cells (lane 2) using Vimentin (D21H3) XP® Rabbit mAb #5741 (upper) or β-Actin (13E5) Rabbit mAb #4970 (lower). The absence of signal in the Vimentin knockout HeLa cells confirms specificity of the antibody for Vimentin.

Cytoskeletal Marker Antibody Sampler Kit: Image 35

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using Pan-Keratin (C11) mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 36

Immunohistochemical analysis of paraffin-embedded human prostate adenocarcinoma using Pan-Keratin (C11) mouse mAb.

Cytoskeletal Marker Antibody Sampler Kit: Image 37

Western blot analysis of extracts from various cell lines, using Pan-Keratin (C11) Mouse mAb.

To Purchase # 8614T
Product # Size Price
8614T
1 Kit  (6 x 20 µl) $ 445

Product Includes Quantity Applications Reactivity MW(kDa) Isotype
Desmin (D93F5) XP® Rabbit mAb 5332 20 µl
  • WB
  • IF
H M R 53 Rabbit IgG
Keratin 17 (D73C7) Rabbit mAb 4543 20 µl
  • WB
  • IF
  • F
H M R 48 Rabbit IgG
Pan-Keratin (C11) Mouse mAb 4545 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 46-58 Mouse IgG1
β-Tubulin (9F3) Rabbit mAb 2128 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk Z B 55 Rabbit IgG
Vimentin (D21H3) XP® Rabbit mAb 5741 20 µl
  • WB
  • IHC
  • IF
  • F
H M R Mk 57 Rabbit IgG
β-Actin (D6A8) Rabbit mAb 8457 20 µl
  • WB
  • IF
  • F
H M R Mk Dm Z 45 Rabbit IgG
Anti-rabbit IgG, HRP-linked Antibody 7074 100 µl
  • WB
Goat 
Anti-mouse IgG, HRP-linked Antibody 7076 100 µl
  • WB
Horse 

Product Description

The Cytoskeletal Marker Antibody Sampler Kit provides an economical means to evaluate the presence and status of select cytoskeleton associated proteins. The kit provides enough primary antibodies to perform two western blots per primary antibody.

Specificity / Sensitivity

Desmin (D93F5) XP® Rabbit mAb, Keratin 17 (D73C7) Rabbit mAb, β-Tubulin (9F3) Rabbit mAb, and Vimentin (D21H3) XP® Rabbit mAb, and B-Actin (D68) Rabbit mAb recognize endogenous levels of total respective target proteins. Pan-Keratin (C11) Mouse mAb detects endogenous levels of total keratins 4, 5, 6, 8, 10, 13, and 18. This antibody does not cross-react with other keratins.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide cooresponding to residues near the amino terminus of human β-actin protein, carboxy terminal residues of human desmin protein, residues near the carboxy terminus of human keratin 17 protein, the amino terminus of human β-tubulin protein, or residues surrounding Arg45 of human vimentin protein. Mouse monoclonal antibody is produced by immunizing animals with a cytoskeleton preparation from A-431 cells.

Background

The cytoskeleton consists of three different types of cystosolic fibers: microtubules, microfilaments (actin) and intermediate filaments. Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). Major types of intermediate filaments are distinguished in part by the tissue in which they are expressed, for example; cytokeratins (epithelial cells), vimentin (mesenchyme origin), and desmin (skeletal, visceral and certain vascular smooth muscle cells) (2). Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form intermediate filaments (3). Research studies have demonstrated that vimentin is present in sarcomas, but not carcinomas, and its expression is examined relative to other markers in order to distinguish between the two forms of neoplasm (4). Desmin is a myogenic marker expressed in early development that forms a network of filaments that extends across the myofibril and surrounds Z discs (5). α/β-tubulin heterodimers form the tubulin subunit that comprises the microtubule building block (6).

.

  1. Herman, I.M. (1993) Curr Opin Cell Biol 5, 48-55.
  2. Eng, L.F. et al. (2000) Neurochem Res 25, 1439-51.
  3. Moll, R. et al. (1982) Cell 31, 11-24.
  4. Leader, M. et al. (1987) Histopathology 11, 63-72.
  5. Li, Z. et al. (1996) Dev Biol 175, 362-6.
  6. Westermann, S. and Weber, K. (2003) Nat Rev Mol Cell Biol 4, 938-47.

Limited Uses

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Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST's products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST's Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

For Research Use Only. Not For Use In Diagnostic Procedures.
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