WB, IP, IHC-P, IF-IC
H M R
Endogenous
96
Rabbit IgG
#P98082
1601
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:1600 - 1:3200 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly388 of human Dab2 protein.
Background
Disabled homolog 2 (Dab2) is a mitogen responsive phosphoprotein that exerts multiple functions through association with numerous proteins. Dab2 modulates signaling pathways through interactions with proteins such as Smads and TGF-β receptors (1,2), axin (3), GRB (4), and Src (5). Dab2 also serves as a cargo-specific adaptor of clathrin-mediated endocytosis via interaction with clathrin (6), AP2 (7), NPXY-containing cargo (8-10), and myosin VI (11,12). In addition, Dab2 regulates cell adhesion by directly binding integrins (13,14). The diverse functions of Dab2 enable it to coordinate cell adhesion, cell motility, membrane trafficking, and signaling. Research studies have shown Dab2 is downregulated in a number of cancers, thereby suggesting a role as a tumor suppressor (15-17). Phosphorylation of Dab2 decreases its endocytotic function (18).
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- Hocevar, B.A. et al. (2005) J Biol Chem 280, 25920-7.
- Hocevar, B.A. et al. (2003) EMBO J 22, 3084-94.
- Xu, X.X. et al. (1998) Oncogene 16, 1561-9.
- Zhou, J. et al. (2003) J Biol Chem 278, 6936-41.
- Mishra, S.K. et al. (2002) EMBO J 21, 4915-26.
- Morris, S.M. and Cooper, J.A. (2001) Traffic 2, 111-23.
- Keyel, P.A. et al. (2006) Mol Biol Cell 17, 4300-17.
- Maurer, M.E. and Cooper, J.A. (2006) J Cell Sci 119, 4235-46.
- Maurer, M.E. and Cooper, J.A. (2005) J Cell Sci 118, 5345-55.
- Morris, S.M. et al. (2002) Traffic 3, 331-41.
- Hasson, T. (2003) J Cell Sci 116, 3453-61.
- Huang, C.L. et al. (2006) J Cell Sci 119, 4420-30.
- Teckchandani, A. et al. (2009) J Cell Biol 186, 99-111.
- Mok, S.C. et al. (1998) Oncogene 16, 2381-7.
- Anupam, K. et al. (2006) World J Gastroenterol 12, 6041-5.
- Bagadi, S.A. et al. (2007) Breast Cancer Res Treat 104, 277-86.
- Chetrit, D. et al. (2011) J Biol Chem 286, 5392-403.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation IHC-P: Immunohistochemistry (Paraffin) IF-IC: Immunofluorescence (Immunocytochemistry)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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