WB
H M R Mk
Endogenous
49
Rabbit IgG
#Q13838
7919
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro365 of human UAP56 protein.
Background
The UAP56 gene is found in the central MHC region and encodes a member of the DEAD-box family of RNA helicases (1). Also known as DDX39B and BAT1, UAP56 functions as an ATP-dependent splicing factor and RNA helicase in the evolutionary conserved transcription/export (TREX) complex. The TREX complex is recruited to sites of active transcription, where it travels along the length of the gene with RNA polymerase II and exports resulting mRNAs to the cytoplasm (2-8).
DDX39, also known as DDX39A, is a DEAD-box helicase highly homologous to UAP56 and is upregulated in lung squamous cell cancers (9,10). DDX39 has been shown to bind to TRF2 to regulate telomere protection (11). Furthermore, DDX39 is overexpressed in several cancer types, which is associated with poor prognosis (12-15). Both UAP56 and DDX39 are hijacked by various viral replication machineries to enable viral reproduction and mRNA export (16-18). UAP56 and DDX39 have also been implicated in promoting the AR-V7 splice variant in advanced prostate cancers (19).
- Peelman, L.J. et al. (1995) Genomics 26, 210-8.
- Fleckner, J. et al. (1997) Genes Dev 11, 1864-72.
- Strässer, K. et al. (2002) Nature 417, 304-8.
- Custódio, N. et al. (2004) RNA 10, 622-33.
- Kapadia, F. et al. (2006) Gene 384, 37-44.
- Shen, J. et al. (2007) J Biol Chem 282, 22544-50.
- Kota, K.P. et al. (2008) J Cell Sci 121, 1526-37.
- Majerciak, V. et al. (2010) Virology 407, 206-12.
- Sugiura, T. et al. (2007) Exp Cell Res 313, 782-90.
- Sugiura, T. et al. (2007) Cancer Biol Ther 6, 957-64.
- Yoo, H.H. and Chung, I.K. (2011) Aging Cell 10, 557-71.
- Kikuta, K. et al. (2012) J Proteomics 75, 1089-98.
- Kuramitsu, Y. et al. (2013) Anticancer Res 33, 2557-60.
- Kuramitsu, Y. et al. (2013) Anticancer Res 33, 3133-6.
- Xing, C. et al. (2020) Front Oncol 10, 1261.
- Dufu, K. et al. (2010) Genes Dev 24, 2043-53.
- Zielke, B. et al. (2011) J Virol 85, 1804-19.
- Wisskirchen, C. et al. (2011) J Virol 85, 8646-55.
- Nakata, D. et al. (2017) Biochem Biophys Res Commun 483, 271-276.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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