WB, IP, FC-FP
H
Endogenous
14-45
Rabbit IgG
#Q9BXN2
64581
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Dectin-1 protein.
Background
Dectin-1, also known as Clec7a, is a C-type lectin receptor expressed by macrophages, monocytes, dendritic cells, neutrophils, and a subset of γδ T cells (1,2). Dectin-1 is a glycoprotein with eight different isoforms, generated through alternative splicing (3-5). It plays an important role in anti-fungal immunity by acting as a pattern recognition receptor for β-glucans found on the cell wall of fungi and some bacteria (5,6). Dectin-1 is composed of a short amino-terminal cytoplasmic domain containing an ITAM-like motif, a transmembrane domain, and an extracellular carboxy-terminal C-type lectin domain (5). Dectin-1 recognizes β-glucans through its C-type lectin domain and transduces signals through its ITAM-like motif by recruiting and activating Syk (7,8). Dendritic cells activated through Dectin-1 promote differentiation of Th17 cells by producing IL-6 and IL-23 (9).
- Taylor, P.R. et al. (2002) J Immunol 169, 3876-82.
- Martin, B. et al. (2009) Immunity 31, 321-30.
- Ariizumi, K. et al. (2000) J Biol Chem 275, 20157-67.
- Kato, Y. et al. (2006) Biol Pharm Bull 29, 1580-6.
- Willment, J.A. et al. (2001) J Biol Chem 276, 43818-23.
- Brown, G.D. and Gordon, S. (2001) Nature 413, 36-7.
- Rogers, N.C. et al. (2005) Immunity 22, 507-17.
- Underhill, D.M. et al. (2005) Blood 106, 2543-50.
- LeibundGut-Landmann, S. et al. (2007) Nat Immunol 8, 630-8.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation FC-FP: Flow Cytometry (Fixed/Permeabilized)
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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