Revision 1
#13962
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For Research Use Only. Not for Use in Diagnostic Procedures.
Applications:
W
Reactivity:
H M R Mk
Sensitivity:
Endogenous
MW (kDa):
50
Source/Isotype:
Rabbit IgG
UniProt ID:
#P35659
Entrez-Gene Id:
7913
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity/Sensitivity
Species predicted to react based on 100% sequence homology
Source / Purification
Background
The DEK proto-oncogene functions as a negative regulator of cellular differentiation, senescence, and apoptosis. DEK is translocated and/or over-expressed in a number of different cancers, including acute myeloid leukemia, breast cancer, cervical cancer, hepatocellular carcinoma, melanoma, and small cell lung cancer (1,2). In addition to the role of DEK in cancer biology, which is mainly related to its intracellular functions, extracellular DEK is implicated in the pathogenesis of autoimmune disorders (1,2). Circulating autoantibodies to DEK have been identified in the serum of patients with autoimmune diseases, including juvenile idiopathic arthritis, sarcoidosis, and systemic lupus erythematosus. DEK is secreted by human monocyte-derived macrophages and apoptotic T-lymphocytes and can act as a chemotactic, pro-inflammatory factor (7,8). Exogenous DEK can penetrate neighboring cells, and translocate to the nucleus to carry out its endogenous nuclear functions (9). IL-8 induced secretion of DEK from macrophages serves as a chemoattractant for peripheral blood leukocytes (7).
Background References
- Broxmeyer, H.E. et al. (2013) Stem Cells 31, 1447-53.
- Riveiro-Falkenbach, E. and Soengas, M.S. (2010) Clin Cancer Res 16, 2932-8.
- Kappes, F. et al. (2011) Genes Dev 25, 673-8.
- Sammons, M. et al. (2006) J Biol Chem 281, 26802-12.
- McGarvey, T. et al. (2000) J Cell Biol 150, 309-20.
- Soares, L.M. et al. (2006) Science 312, 1961-5.
- Mor-Vaknin, N. et al. (2006) Mol Cell Biol 26, 9484-96.
- Kappes, F. et al. (2008) Mol Cell Biol 28, 3245-57.
- Saha, A.K. et al. (2013) Proc Natl Acad Sci U S A 110, 6847-52.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
W: Western Blotting
Cross-Reactivity Key
H: Human M: Mouse R: Rat Mk: Monkey
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