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DGAT1 (F7A3Z) Rabbit Monoclonal Antibody #27469

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  • IF

    Product Specifications

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 49
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IF-Immunofluorescence 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Simple Western™ 1:50 - 1:250
    Immunofluorescence (Immunocytochemistry) 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DGAT1 (F7A3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total DGAT1 protein. This antibody does not cross-react with DGAT2 protein. Faint nuclear background may be observed by immunofluorescence.

    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val31 of human DGAT1 protein.

    Background

    Diacylglycerol O-acyltransferase 1 (DGAT1) catalyzes triacylglycerol biosynthesis from diacylglycerol and fatty acyl-CoA (1). Studies show that DGAT1 is highly expressed in glioblastoma (GBM), stimulating the storage of excess fatty acids in triacylglycerols and lipid droplets to block GBM cancer cells from oxidative damage. DGAT1 inhibition in GBM cancer cells enhances reactive oxygen species (ROS) production and causes mitochondrial damage, leading to cancer cell death (2). In addition, Jumonji domain-containing 6 (JMJD6) is critical for clear cell renal cell carcinoma (ccRCC) development. JMJD6, along with RBM39, promotes DGAT1 expression. DGAT1 inhibition suppresses ccRCC tumorigenesis in vivo (3).

    Alternate Names

    ACAT related gene product 1; ACAT-related gene product 1; acyl coenzyme A:cholesterol acyltransferase related gene 1; Acyl-CoA retinol O-fatty-acyltransferase; acyl-CoA:diacylglycerol acyltransferase; AGRP1; ARAT; ARGP1; DGAT; DGAT1; Diacylglycerol O-acyltransferase 1; diacylglycerol O-acyltransferase homolog 1 (mouse); DIAR7; Diglyceride acyltransferase; Retinol O-fatty-acyltransferase

    For Research Use Only. Not for Use in Diagnostic Procedures.
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