WB, IP
H
Endogenous
80
Rabbit IgG
#P23743
1606
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu734 of human DGKA protein.
Background
Diacylglycerol kinase alpha (DGKA) is a type 1 diacylglycerol kinase that competes with protein kinase C to convert messenger diacylglycerol (DAG) to phosphatidic acid (1). Upon activation of this intracellular lipid kinase, DGKA rapidly relocates from the cytosol to the plasma membrane where it is involved in multiple cellular processes, including regulating lipid and glucose metabolism levels, cell growth, and cell signaling (2,3). Expression of DGKA is high in T lymphocytes, and detectable in endothelial cells, epithelial cells, fibroblasts, and oligodendrocytes (4). DGKA negatively regulates T cell receptor-dependent responses through phosphorylation of phospholipase C-derived DAG (5-7). In contrast, DGKA-dependent phosphatidic acid generation by phosphorylation of a pre-existing DAG pool is necessary for IL-2-dependent T cell proliferation (8). DGKA is frequently overexpressed in cancer, promoting tumor cell survival, proliferation, invasion, and metastasis (9-12). Due to its role in immunity and cancer, targeting DGKA represents a unique opportunity for therapeutic intervention in cancer (13).
- Mérida, I. et al. (2008) Biochem J 409, 1-18.
- Xie, S. et al. Cell Logist 5, e1078431.
- Kelly, E. et al. (2018) Dis Model Mech 11, dmm035600. doi: 10.1242/dmm.035600.
- Baldanzi, G. et al. (2004) Oncogene 23, 4828-38.
- Díaz-Flores, E. et al. (2003) J Biol Chem 278, 29208-15.
- Prinz, P.U. et al. (2012) J Immunol 188, 5990-6000.
- Alonso, R. et al. (2011) Cell Death Differ 18, 1161-73.
- Flores, I. et al. (1999) J Immunol 163, 708-14.
- Yanagisawa, K. et al. (2007) Biochim Biophys Acta 1771, 462-74.
- Bacchiocchi, R. et al. (2005) Blood 106, 2175-82.
- Takeishi, K. et al. (2012) J Hepatol 57, 77-83.
- Baldanzi, G. et al. (2011) Cancer Sci 102, 1329-36.
- Purow, B. (2015) Clin Cancer Res 21, 5008-12.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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