WB, IP
H M R Mk
Endogenous
19
Rabbit
#Q01658
1810
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Species predicted to react based on 100% sequence homology
The antigen sequence used to produce this antibody shares
100% sequence homology with the species listed here, but
reactivity has not been tested or confirmed to work by CST.
Use of this product with these species is not covered under
our
Product Performance Guarantee.
D. melanogaster, Zebrafish, Dog, Pig
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly112 of human DR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Down-regulator of transcription 1 (DR1), also known as negative cofactor 2-β (NC2-β), forms a heterodimer with DR1 associated protein 1 (DRAP1)/NC2-α and acts as a negative regulator of RNA polymerase II and III (RNAPII and III) transcription (1-5). DR1 activity is thought to be important for modulating the switch between basal transcription activity and transcription activator driven transcription (2,6,7). DR1 interaction with TATA binding protein (TBP) blocks the association of general transcription factors TFIIA and TFIIB with TBP and disrupts the formation of the RNAPII transcription initiation complex (1,8,9). RNAPIII driven transcription is also inhibited by DR1 interaction with TBP. DR1 disrupts the interaction of TBP with the TFIIB related factor (BRF)/RNAPIII B-related factor, inhibiting transcription initiation by the RNAPIII machinery (4).
- Inostroza, J.A. et al. (1992) Cell 70, 477-89.
- Meisterernst, M. and Roeder, R.G. (1991) Cell 67, 557-67.
- Mermelstein, F. et al. (1996) Genes Dev 10, 1033-48.
- White, R.J. et al. (1994) Science 266, 448-50.
- Kantidakis, T. and White, R.J. (2010) Nucleic Acids Res 38, 1228-39.
- Kraus, V.B. et al. (1994) Proc Natl Acad Sci U S A 91, 6279-82.
- Yeung, K.C. et al. (1994) Genes Dev 8, 2097-109.
- Kim, T.K. et al. (1995) J Biol Chem 270, 10976-81.
- Kamada, K. et al. (2001) Cell 106, 71-81.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
Limited Uses
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