WB, IP
H M R Mk
Endogenous
132
Rabbit
#Q16643
1627
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala330 of mouse Drebrin A protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Developmentally-regulated brain proteins (Drebrins) are cytoplasmic proteins that were originally identified in the brain as F-actin-binding proteins. There are two mammalian isoforms: adult type (A) and embryonic type (E). These isoforms are derived from a single gene through alternative RNA splicing mechanisms (1). Drebrin E has been observed to accumulate in the developmental stage of migrating neurons and in the growing cell processes of neurons. Drebrin A is found at the dendritic spines of mature cortical neurons where it plays a role in synaptic plasticity (2,3). Although drebrins are primarily found in neurons, they have also been found in skeletal muscle, heart, pancreas, and kidney. Research studies have shown that reduced expression of drebrin in the brain could be associated with Alzheimer’s Disease, Down Syndrome (4), and bipolar disorders (5).
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting IP: Immunoprecipitation
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
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