WB
H
Endogenous
58
Rabbit
#Q9UJU6
28988
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly147 of human Drebrin-like protein/DBNL. Antibodies are purified by peptide affinity chromatography.
Background
Drebrin-like protein/DBNL is an actin-binding protein that expresses in the developing cerebral cortex and contributes to the formation and morphology of processes in the multipolar neurons. The expression of Drebrin-like protein increases the levels of N-cadherin, promoting intracellular trafficking and cell migration. High expression of N-cadherin can rescue deficits in cell migration induced by a defective Drebrin-like protein in neurons (1,2). Drebrin-like protein directly interacts with Cobl, forming a complex found in Purkinje cell bodies and dendritic arbors in the cerebellum (3). Severe impairments in the recycling of synaptic vesicles in hippocampal neurons and behavioral anomalies occur in mice deficient of Drebrin-like protein (4). The phosphorylation of Drebrin-like protein at Ser269/Thr291 promotes the localization in the cortical plate of the Golgi apparatus, facilitating the correct intracellular protein trafficking (5). Drebrin-like protein participates in regulatory network signaling by the activation of β-adrenergic receptors during the proliferation of cardiac fibroblasts, which is associated with the extracellular activation of ERK (4). Drebrin-like protein plays a role in cell migration, the extension of neuronal processes and plasticity of dendrites, and is required for the polymerization of actin at immunological synapses (IS), for CXCR4 recruitment to IS, and activation of T-cells (5-7).
- Inoue, S. et al. (2019) J Neurosci 39, 678-91.
- Rocha-Perugini, V. et al. (2017) Adv Exp Med Biol 1006, 271-80.
- Haag, N. et al. (2012) J Neurosci 32, 17842-56.
- Connert, S. et al. (2006) EMBO J 25, 1611-22.
- Li, Z. et al. (2014) Oncotarget 5, 3197-209.
- Liu, N. et al. (2014) Mol Biosyst 10, 1932-9.
- Le Bras, S. et al. (2004) J Biol Chem 279, 15550-60.
Species Reactivity
Species reactivity is determined by testing in at least one approved application (e.g., western blot).
Western Blot Buffer
IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v BSA, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.
Applications Key
WB: Western Blotting
Cross-Reactivity Key
H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected
Trademarks and Patents
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